Tumpa Naz Fathma, Kang Mingyeong, Yoo Jiae, Kim Sunju, Kwak Minseok
Department of Chemistry, Pukyong National University, Busan 48513, Korea.
Industry 4.0 Convergence Bionics Engineering, New-Senior' Oriented Smart Health Care Education Center, Pukyong National University, Busan 48513, Korea.
Bioengineering (Basel). 2022 Apr 11;9(4):170. doi: 10.3390/bioengineering9040170.
In the era of climate changes, harmful dinoflagellate outbreaks that produce potent algal toxins, odor, and water discoloration in aquatic environments have been increasingly reported. Thus, various treatments have been attempted for the mitigation and management of harmful blooms. Here, we report engineered nanoparticles that consist of two different types of rylene derivatives encapsulated in polymeric micelles. In addition, to avoid dissociation of the aggregate, the core of micelle was stabilized via semi-interpenetrating network (sIPN) formation. On two types of the marine red-tide dinoflagellates, and , the nanoparticle uptake followed by fluorescence labeling and photothermal effect was conducted. Firstly, fluorescence microscopy enabled imaging of the dinoflagellates with the ultraviolet chromophore, Lumogen Violet. Lastly, near-infrared (NIR) laser irradiation was exposed on the Lumogen IR788 nanoparticle-treated The irradiation resulted in reduced cell survival due to the photothermal effect in microalgae. The results suggested that the nanoparticle, IR788-sIPN, can be applied for potential red-tide algal elimination.
在气候变化时代，水生环境中产生强效藻毒素、异味和水体变色的有害甲藻爆发的报道日益增多。因此，人们尝试了各种处理方法来减轻和管理有害藻华。在此，我们报道了一种工程化纳米颗粒，它由包裹在聚合物胶束中的两种不同类型的苝衍生物组成。此外，为避免聚集体解离，通过半互穿网络（sIPN）的形成使胶束核心得以稳定。在两种海洋赤潮甲藻，即[甲藻名称1]和[甲藻名称2]上，进行了纳米颗粒摄取，随后进行荧光标记和光热效应实验。首先，荧光显微镜能够利用紫外发色团鲁米诺紫对甲藻进行成像。最后，对经鲁米诺红外788纳米颗粒处理的[甲藻名称]进行近红外（NIR）激光照射。由于微藻中的光热效应，照射导致细胞存活率降低。结果表明，纳米颗粒IR788-sIPN可用于潜在的赤潮藻类消除。
