乙肝病毒转基因小鼠中乙肝病毒感染的代谢调控

Metabolic Regulation of Hepatitis B Virus Infection in HBV-Transgenic Mice.

作者信息

Lan Wenning, Wang Yang, Zhou Zixiong, Sun Xia, Zhang Yun, Zhang Fangrong

机构信息

Key Laboratory of Gastrointestinal Cancer, Ministry of Education, School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350122, China.

Xiamen Key Laboratory of Rare Earth Photoelectric Functional Materials, Xiamen Institute of Rare Earth Materials, Haixi Institute, Chinese Academy of Sciences, Xiamen 361021, China.

出版信息

Metabolites. 2022 Mar 25;12(4):287. doi: 10.3390/metabo12040287.

DOI:10.3390/metabo12040287

PMID:35448475

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9031567/

Abstract

Hepatitis B virus (HBV) infection is a worldwide health burden. Metabolomics analysis has revealed HBV-induced metabolism dysregulation in liver tissues and hepatocytes. However, as an infectious disease, the tissue-specific landscape of metabolic profiles of HBV infection remains unclear. To fill this gap, we applied untargeted nuclear magnetic resonance (NMR) metabolomic analysis of the heart, liver, spleen, lung, kidney, pancreas, and intestine (duodenum, jejunum, ileum) in HBV-transgenic mice and their wild-type littermates. Strikingly, we found systemic metabolic alterations induced by HBV in liver and extrahepatic organs. Significant changes in metabolites have been observed in most tissues of HBV-transgenic mice, except for ileum. The metabolic changes may provide novel therapeutic targets for the treatment of HBV infection. Moreover, tissue-specific metabolic profiles could speed up the study of HBV induced systemic metabolic reprogramming, which could help follow the progression of HBV infection and explain the underlying pathogenesis.

摘要

乙型肝炎病毒（HBV）感染是一项全球性的健康负担。代谢组学分析已揭示HBV诱导肝脏组织和肝细胞中的代谢失调。然而，作为一种传染病，HBV感染的组织特异性代谢谱格局仍不清楚。为了填补这一空白，我们对HBV转基因小鼠及其野生型同窝小鼠的心脏、肝脏、脾脏、肺、肾脏、胰腺和肠道（十二指肠、空肠、回肠）进行了非靶向核磁共振（NMR）代谢组学分析。令人惊讶的是，我们发现HBV在肝脏和肝外器官中诱导了全身性代谢改变。除回肠外，在HBV转基因小鼠的大多数组织中均观察到代谢物的显著变化。这些代谢变化可能为HBV感染的治疗提供新的治疗靶点。此外，组织特异性代谢谱可以加速对HBV诱导的全身性代谢重编程的研究，这有助于追踪HBV感染的进展并解释潜在的发病机制。