使用化学成分明确的培养基实现快速高效的小鼠多能性重编程

Fast and Efficient Mouse Pluripotency Reprogramming Using a Chemically-Defined Medium.

作者信息

Huang Junju, Yang Xuejie, Wang Jie, Wu Haoyu, Pei Duanqing, Chen Jiekai

机构信息

Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical University, Guangzhou 511436, China.

CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.

出版信息

Methods Protoc. 2022 Mar 24;5(2):28. doi: 10.3390/mps5020028.

DOI:10.3390/mps5020028

PMID:35448693

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9027986/

Abstract

The reprogramming of somatic cells to obtain induced pluripotent stem cells (iPSCs) is an important biological and medical breakthrough, providing important applications for fields such as regenerative medicine and disease modeling. However, this promising technology is damped due to its low efficiency and slow kinetics. Therefore, we generated a practical workflow to rapidly and efficiently induce iPSCs from mouse embryonic fibroblasts (MEFs) using iCD1 (iPS chemically-defined medium 1). This protocol can easily be implemented in a standard cell culture laboratory and be applied to cell fate research.

摘要

将体细胞重编程以获得诱导多能干细胞（iPSC）是一项重要的生物学和医学突破，为再生医学和疾病建模等领域提供了重要应用。然而，这项前景广阔的技术因其效率低和动力学缓慢而受到限制。因此，我们开发了一种实用的工作流程，使用iCD1（iPS化学定义培养基1）从小鼠胚胎成纤维细胞（MEF）中快速高效地诱导iPSC。该方案可在标准细胞培养实验室轻松实施，并应用于细胞命运研究。