TCDD 暴露改变雌雄小鼠粪便 IgA 浓度。
TCDD exposure alters fecal IgA concentrations in male and female mice.
机构信息
School of Biological Sciences, College of Natural and Health Sciences, University of Northern Colorado, Greeley, CO, 80639, USA.
Department of Computer Science, College of Engineering and Applied Science, University of Colorado, Boulder, 80309, CO, USA.
出版信息
BMC Pharmacol Toxicol. 2022 Apr 21;23(1):25. doi: 10.1186/s40360-022-00563-9.
BACKGROUND
Activation of the aryl hydrocarbon receptor (AhR) can alter diurnal rhythms including those for innate lymphoid cell numbers, cytokine and hormone levels, and feeding behaviors. Because immune responses and antibody levels are modulated by exposure to AhR agonists, we hypothesized that some of the variation previously reported for the effects of AhR activation on fecal secretory immunoglobulin A (sIgA) levels could be explained by dysregulation of the diurnal sIgA rhythm.
METHODS
C57Bl/6 J mice were exposed to peanut oil or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, 10 or 40 μg/Kg) and fecal sIgA levels were determined in samples collected every 4 h over 4 days.
RESULTS
Fecal sIgA concentrations were not significantly different between light and dark phases of the photoperiod in either male or female mice, and there were no significant circadian rhythms observed, but TCDD exposure significantly altered both fecal mesor sIgA and serum IgA concentrations, in parallel, in male (increased) and female (biphasic) mice.
CONCLUSIONS
AhR activation can contribute to the regulation of steady state IgA/sIgA concentrations.
背景
芳基烃受体 (AhR) 的激活可以改变昼夜节律，包括固有淋巴细胞数量、细胞因子和激素水平以及进食行为的昼夜节律。由于免疫反应和抗体水平受到 AhR 激动剂的调节，我们假设先前报道的 AhR 激活对粪便分泌型免疫球蛋白 A (sIgA) 水平的影响的一些变化可以通过昼夜节律 sIgA 节律的失调来解释。
方法
将 C57Bl/6J 小鼠暴露于花生油或 2,3,7,8-四氯二苯并对二恶英 (TCDD，10 或 40μg/kg)，并在 4 天内每隔 4 小时收集粪便样本以测定粪便 sIgA 水平。
结果
无论雄性还是雌性小鼠，粪便 sIgA 浓度在光暗周期的明暗阶段均无显着差异，也未观察到明显的昼夜节律，但 TCDD 暴露显着改变了雄性（增加）和雌性（双相）小鼠的粪便均数 sIgA 和血清 IgA 浓度。
结论
AhR 激活可以有助于调节稳态 IgA/sIgA 浓度。
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