Calamera J C, Quiros M C, Brugo S, Nicholson R F, Vilar O
Andrologia. 1986 Nov-Dec;18(6):574-80. doi: 10.1111/j.1439-0272.1986.tb01832.x.
Fertility of men depends on the quality of semen. The aim of the present paper is to determine both the acrosin activity by radioimmunoassay and ATP concentration by bioluminescence in human spermatozoa, and evaluate these results in those samples with normal or low sperm penetration according to SPA test. Ejaculates obtained from 42 untreated men, were studied one hour after the obtention. These materials were divided into two groups:20 human semen samples with "in vitro" potentiality to penetrate zone-free hamster ova, between 15% to 98% and 22 human semen with SPA test between 0% to 14%. When we compare the group with normal penetration response vs that group with low or absent penetration ones, a significant decrease of ATP and acrosin concentrations was observed (P less than 0.001). Nevertheless no significant difference was observed in relation with percentage of motility, volume (ml), sperm concentration (10(6)/ml), percent of quick progressive spermatozoa and number of gametes capable of migrating into the medium layer (10(6)), between the group with low or absent penetration test against that one with normal zona-free hamster egg test.
男性的生育能力取决于精液质量。本文旨在通过放射免疫分析法测定人类精子中的顶体蛋白酶活性，并通过生物发光法测定ATP浓度，并根据精子穿透试验（SPA），对精子穿透正常或低下的样本的这些结果进行评估。从42名未经治疗的男性获取的射精样本在获取后1小时进行研究。这些样本被分为两组：20份人类精液样本，其“体外”穿透去透明带仓鼠卵的能力在15%至98%之间；22份人类精液样本的SPA试验结果在0%至14%之间。当我们比较穿透反应正常的组与穿透低下或无穿透的组时，观察到ATP和顶体蛋白酶浓度显著降低（P<0.001）。然而，在穿透试验低下或无穿透的组与去透明带仓鼠卵试验正常的组之间，在活力百分比、体积（ml）、精子浓度（10⁶/ml）、快速前进精子百分比以及能够迁移到中间层的配子数量（10⁶）方面，未观察到显著差异。
