Obstetrics and Gynecology Department, the 8th People's Hospital of Shanghai, Shanghai, China.
Obstetrics and Gynecology Department, the 6th People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China.
J Obstet Gynaecol Res. 2022 Jul;48(7):1613-1620. doi: 10.1111/jog.15256. Epub 2022 Apr 22.
To identify trophoblastic cells retrieved from the cervix at a gestational age (GA) of 5-9 weeks by a noninvasive modality in fetuses.
Transcervical cells (TCCs) were noninvasively extracted by a cytobrush using the Papanicolaou sampling method. TCCs were immunostained with antihuman leukocyte antigen (HLA)-G and anticytokeratin (CK)-7 antibodies to identify trophoblastic cells. Maternal finger blood, gestational sacs, and 20 trophoblastic cells collected by a laser-guided microscopic single-cell capture system were examined and compared by short tandem repeat (STR) genotyping.
Forty-nine pregnant women with GA of 5-9 weeks and six nonpregnant healthy women were included in the study. Trophoblastic cells were identified in 37 (75.5%) TCC samples, among which 34 (69.4%) were eligible for STR genotyping analysis. No trophoblastic cells were identified in nonpregnant healthy women. The STR genotyping analyses revealed 24 female and 10 male fetuses. TCC trophoblastic cells exhibited the same STR profiles as gestational sac and maternal blood in all samples, which indicated that the TCC trophoblastic cells originated from fetuses.
This primary study validated that trophoblastic cells from TCCs at GA 5-9 weeks originated from the fetus. Further studies are needed to verify whether this method can be used for early noninvasive prenatal diagnosis and paternity testing.
鉴定妊娠 5-9 周胎儿经宫颈用非侵入性方法获取的滋养层细胞。
采用巴氏涂片法用细胞刷经宫颈非侵入性提取宫颈细胞（TCC）。用抗人白细胞抗原（HLA）-G 和抗细胞角蛋白（CK）-7 抗体对 TCC 进行免疫染色，以鉴定滋养层细胞。对母指血、妊娠囊和激光引导的显微镜单细胞捕获系统收集的 20 个滋养层细胞进行短串联重复序列（STR）基因分型检测，并进行比较。
本研究纳入了 49 名妊娠 5-9 周的孕妇和 6 名非妊娠健康女性。在 37 份（75.5%）TCC 样本中鉴定出滋养层细胞，其中 34 份（69.4%）可进行 STR 基因分型分析。非妊娠健康女性未鉴定出滋养层细胞。STR 基因分型分析显示 24 名女性和 10 名男性胎儿。所有样本中 TCC 滋养层细胞的 STR 图谱与妊娠囊和母血相同，这表明 TCC 滋养层细胞来源于胎儿。
本初步研究验证了妊娠 5-9 周 TCC 中的滋养层细胞来源于胎儿。需要进一步研究以验证该方法是否可用于早期非侵入性产前诊断和亲子关系鉴定。
