College of Basic Medical Sciences, Dalian Medical University, Dalian, China.
School of Stomatology, Dalian Medical University, Dalian, China.
Oral Dis. 2023 Jul;29(5):2154-2162. doi: 10.1111/odi.14218. Epub 2022 May 3.
Exploring the role of OPN N-glycosylation in osteoblasts and osteoclasts.
Immunohistochemistry was used to detect the expression of OPN in mice with apical periodontitis. The asparagine at position 79 of the OPN protein was mutated to glutamine, and the above plasmids were transfected into osteoblasts and osteoclasts. The effect of OPN N-glycosylation on proliferation of osteoblasts and osteoclasts was detected by CCK8 assays. Western blotting was used to detect the expression of OPN N-glycosylation on osteoclasts and osteoblasts. Detection of N-glycosylation of OPN activated the NF-κB signaling pathway to regulate osteoblasts and osteoclasts.
OPN increased the expression in a mice model of apical periodontitis. The expression curve of OPN resembled a reverse V shape. The OPN N-glycosylation site was identified as 79 by MS. N-glycosylation of OPN promoted the proliferation of osteoclasts. But the N79 glycosylation site of mutant OPN could not increase the proliferation of osteoblasts. OPN N-glycosylation modulated the expression of osteoclast- and osteoblast-associated factors through the NF-κB signaling pathway. N-glycosylation of OPN promoted nuclear translocation of NF-κB in osteoclasts and osteoblasts.
The N-glycosylation site of OPN is 79. N-glycosylation of OPN played an important role in the biological function of OPN protein.
探索 OPN N-糖基化在成骨细胞和破骨细胞中的作用。
采用免疫组织化学方法检测根尖周炎小鼠 OPN 的表达。将 OPN 蛋白第 79 位天冬酰胺突变为谷氨酰胺，将上述质粒转染至成骨细胞和破骨细胞。通过 CCK8 法检测 OPN N-糖基化对成骨细胞和破骨细胞增殖的影响。Western blot 检测 OPN N-糖基化在破骨细胞和成骨细胞中的表达。检测 OPN 的 N-糖基化激活 NF-κB 信号通路对成骨细胞和破骨细胞的调节作用。
OPN 在根尖周炎小鼠模型中的表达增加。OPN 的表达曲线呈反 V 形。MS 鉴定 OPN 的 N-糖基化位点为 79。OPN 的 N-糖基化促进破骨细胞的增殖。但突变型 OPN 的 N79 糖基化位点不能增加成骨细胞的增殖。OPN N-糖基化通过 NF-κB 信号通路调节破骨细胞和成骨细胞相关因子的表达。OPN 的 N-糖基化促进 NF-κB 在破骨细胞和成骨细胞中的核转位。
OPN 的 N-糖基化位点为 79。OPN 的 N-糖基化在 OPN 蛋白的生物学功能中起着重要作用。
