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通过500兆赫质子核磁共振和奥弗豪泽效应增强技术对酵母核糖体5.8S RNA中富含鸟嘌呤-胞嘧啶的共同臂进行验证。

Demonstration of the GC-rich common arm in yeast ribosomal 5.8S RNA via 500-MHz proton nuclear magnetic resonance and Overhauser enhancements.

作者信息

Lee K M, Marshall A G

出版信息

Biochemistry. 1986 Dec 16;25(25):8245-52. doi: 10.1021/bi00373a018.

DOI:10.1021/bi00373a018
PMID:3545290
Abstract

In this paper we report the first 1H NMR study of the base-paired secondary structure of yeast 5.8S RNA. On the basis of a combination of homonuclear Overhauser enhancements and temperature dependence of the proton 500-MHz NMR spectrum, we are able to identify and assign eight of the nine base pairs in the most thermally stable helical arm: G116.C137-C117.G136-C118.G135- C119.G134-C120.G133-U121.G132- U122.A131-G123.C130. This arm contains an unusually temperature-stable (to 71 degrees C) segment of four consecutive G.C base pairs. This work constitutes the most direct evidence to date for the existence and base-pair sequence of the GC-rich helix, which is common to most currently popular secondary structural models for eukaryotic 5.8S ribosomal RNA.

摘要

在本文中，我们报告了对酵母5.8S RNA碱基配对二级结构的首次1H NMR研究。基于同核Overhauser增强效应与质子500 MHz NMR谱温度依赖性的结合，我们能够识别并确定最耐热螺旋臂中九个碱基对中的八个：G116.C137 - C117.G136 - C118.G135 - C119.G134 - C120.G133 - U121.G132 - U122.A131 - G123.C130。该臂包含一段由四个连续G.C碱基对组成的异常耐热（至71摄氏度）的片段。这项工作构成了迄今为止关于富含GC螺旋的存在及其碱基对序列的最直接证据，这种富含GC的螺旋在目前大多数流行的真核5.8S核糖体RNA二级结构模型中都很常见。

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