Yang Yu Zhu, Qiu Wan Xiang, Xu Zi Yi, Sun Zhe, Qing Min, Li Nian Bing, Luo Hong Qun
Key Laboratory of Luminescence Analysis and Molecular Sensing, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China; Department of Basic Teaching, Zunyi Medical and Pharmaceutical College, Zunyi 563006, PR China.
Key Laboratory of Luminescence Analysis and Molecular Sensing, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Sep 5;277:121254. doi: 10.1016/j.saa.2022.121254. Epub 2022 Apr 14.
A new type of dye with advantages of high selectivity and sensitivity is formed by using the strategy of hybridization between the luminescent unit and recognition unit. Based on this strategy, we exploit a novel dye bonding the benzopyrylium salt as a luminescent unit and phenylboronate group as a response site, which is served as a fluorescent probe 1 for specific recognition of hydrogen peroxide in biological application. Probe 1 employs a unique recognition switch, phenylboronate unit, to"turn-on"a highly specific and rapid fluorescence response toward hydrogen peroxide combined with the 1,6-rearrangement elimination reaction strategy. Meanwhile, probe 1 has the ability to glucose assay by taking advantage of glucose oxidase/glucose enzymatic reaction. What's more, the probe 1 is capable of tracking endogenous hydrogen peroxide in living cells and intracellular imaging. Therefore, the newly developed bioprobe 1 is expected to be used to monitor hydrogen peroxide and glucose levels in complex organisms.
通过使用发光单元与识别单元之间的杂交策略，形成了一种具有高选择性和灵敏度优点的新型染料。基于该策略，我们开发了一种新型染料，它将苯并吡喃鎓盐作为发光单元，苯基硼酸酯基团作为响应位点，在生物应用中用作特异性识别过氧化氢的荧光探针1。探针1采用独特的识别开关——苯基硼酸酯单元，结合1,6-重排消除反应策略，对过氧化氢“开启”高度特异性和快速的荧光响应。同时，探针1利用葡萄糖氧化酶/葡萄糖酶促反应具有检测葡萄糖的能力。此外，探针1能够追踪活细胞中的内源性过氧化氢并进行细胞内成像。因此，新开发的生物探针1有望用于监测复杂生物体中的过氧化氢和葡萄糖水平。
