结直肠癌的临床病理特征与膜结合粘蛋白基因和 LncRNAs 的关系。

The association of clinicopathological characterizations of colorectal cancer with membrane-bound mucins genes and LncRNAs.

机构信息

Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Farhikhtegan Medical Convergence sciences Research Centre, Farhikhtegan Hospital, Islamic Azad University, Tehran Medical Sciences, Tehran, Iran.

Farhikhtegan Medical Convergence sciences Research Centre, Farhikhtegan Hospital, Islamic Azad University, Tehran Medical Sciences, Tehran, Iran; Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

出版信息

Pathol Res Pract. 2022 May;233:153883. doi: 10.1016/j.prp.2022.153883. Epub 2022 Apr 4.

DOI:10.1016/j.prp.2022.153883

PMID:35452967

Abstract

BACKGROUND

Colorectal cancer (CRC) is one of the most common malignancies in the world and has a high mortality rate. It is believed that dysfunction in the expression of mucins and aberrant expression of some lncRNAs are associated with the occurrence and development of CRC. Therefore, the aim of the present study was to investigate the expression of MUC15, MUC16, MUC20, PCAT1, CCAT1 and HOTAIR genes in colorectal cancer and its relationship with clinicopathological variables.

MATERIALS AND METHODS

This research was prospective case-control study. Tumors from CRC patients were collected from the Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran. RNA extraction and cDNA synthesis were performed using the corresponding kits. The gene primer was designed and RT-PCR was used to evaluate gene expression. The t-test and ANOVA were employed to examine the differences between groups. Data analysis was performed using Prism8 software.

RESULTS

The results of the present study showed that the expression of MUC15 (P = 0.0012), MUC20 (P = 0.009) and CCAT1 (P = 0.001) genes in patients with colorectal cancer were significantly different from tumor margin samples. There were also associations between the expression of the studied genes and clinicopathological variables such as grade and stage of colorectal cancer tumor as well as the age of the patients. The area under the curves (AUC) for the MUC15 0.953 (95% CI 7565-0.9897, P = 0.0003), MUC20 0.782 (95% CI 0.6163-0.9482, P = 0.008) and CCAT1 0.917 (95% CI 0.8015-1, P = 0.0003) were calculated by ROC analysis.

CONCLUSION

The current experiment revealed changes in expression level of mucin genes and lncRNAs in CRC and its different stages, showing that they can be considered as biomarkers for diagnosis of this cancer.

摘要

背景

结直肠癌（CRC）是世界上最常见的恶性肿瘤之一，死亡率很高。据信，粘蛋白表达功能障碍和某些长链非编码 RNA 的异常表达与 CRC 的发生和发展有关。因此，本研究旨在探讨粘蛋白基因 MUC15、MUC16、MUC20、PCAT1、CCAT1 和 HOTAIR 在结直肠癌中的表达及其与临床病理变量的关系。

材料和方法

本研究为前瞻性病例对照研究。从伊朗德黑兰谢赫比赫蒂医科大学塔莱加尼医院收集 CRC 患者的肿瘤。使用相应的试剂盒进行 RNA 提取和 cDNA 合成。设计基因引物，采用 RT-PCR 评估基因表达。采用 t 检验和 ANOVA 检验组间差异。数据分析使用 Prism8 软件。

结果

本研究结果表明，MUC15（P=0.0012）、MUC20（P=0.009）和 CCAT1（P=0.001）基因在结直肠癌患者中的表达与肿瘤边缘样本明显不同。研究基因的表达与结直肠癌肿瘤的分级和分期以及患者年龄等临床病理变量也存在关联。MUC15 的曲线下面积（AUC）为 0.953（95%CI 7565-0.9897，P=0.0003），MUC20 的 AUC 为 0.782（95%CI 0.6163-0.9482，P=0.008），CCAT1 的 AUC 为 0.917（95%CI 0.8015-1，P=0.0003），通过 ROC 分析计算得出。