Teng Xin-Nan, Wang Shu-Chang, Zeb Liaqat, Xiu Zhi-Long
School of Bioengineering, Dalian University of Technology, Dalian 116024, PR China.
School of Bioengineering, Dalian University of Technology, Dalian 116024, PR China.
Food Chem. 2022 Sep 15;388:132995. doi: 10.1016/j.foodchem.2022.132995. Epub 2022 Apr 18.
High acid value (AV) and fluorine content of Antarctic krill oil (AKO) extracted from frozen krill by ethanol limit its product development. In this study, a method was proposed to reduce the AV and fluorine content of AKO by carboxymethyl chitosan (CMCS) adsorption. The optimal adsorption condition was 12.5% (w/v) of CMCS at 30℃ for 15 min. At this condition, AV and fluorine content decreased by 78.0% and 61.4%, respectively. It is interesting that CMCS adsorption showed specificity to particular substances. Although free fatty acids content showed a significant reduction, free EPA and DHA, phospholipid and astaxanthin remained almost constant. Moreover, CMCS adsorption showed no influence on neuroprotective activity of AKO against HO-induced neuro-damage of PC12 cells. The reclaimed CMCS showed an undiminished antimicrobial activity against both Gram-positive and Gram-negative bacteria. The CMCS adsorption shows a potential development for refining AKO and other oils in food industry.
通过乙醇从冷冻磷虾中提取的南极磷虾油（AKO）的高酸值（AV）和氟含量限制了其产品开发。在本研究中，提出了一种通过羧甲基壳聚糖（CMCS）吸附降低AKO的AV和氟含量的方法。最佳吸附条件为30℃下12.5%（w/v）的CMCS，吸附15分钟。在此条件下，AV和氟含量分别降低了78.0%和61.4%。有趣的是，CMCS吸附对特定物质具有特异性。尽管游离脂肪酸含量显著降低，但游离EPA和DHA、磷脂和虾青素几乎保持不变。此外，CMCS吸附对AKO对HO诱导的PC12细胞神经损伤的神经保护活性没有影响。回收的CMCS对革兰氏阳性菌和革兰氏阴性菌均表现出不减的抗菌活性。CMCS吸附在食品工业中对精炼AKO和其他油脂具有潜在的开发价值。
