单细胞测序：急性髓系白血病迷宫中的阿里阿德涅之线

Single-Cell Sequencing: Ariadne's Thread in the Maze of Acute Myeloid Leukemia.

作者信息

Redavid Immacolata, Conserva Maria Rosa, Anelli Luisa, Zagaria Antonella, Specchia Giorgina, Musto Pellegrino, Albano Francesco

机构信息

Hematology Section, Department of Emergency and Organ Transplantation (D.E.T.O.), University of Bari 'Aldo Moro', 70124 Bari, Italy.

School of Medicine, University of Bari 'Aldo Moro', 70124 Bari, Italy.

出版信息

Diagnostics (Basel). 2022 Apr 15;12(4):996. doi: 10.3390/diagnostics12040996.

DOI:10.3390/diagnostics12040996

PMID:35454044

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9024495/

Abstract

Acute myeloid leukemia (AML) is a haematological neoplasm resulting from the accumulation of genetic and epigenetic alterations. Patients' prognoses vary with AML genetic heterogeneity, which hampers successful treatments. Single-cell approaches have provided new insights of the clonal architecture of AML, revealing the mutational history from diagnosis, during treatment and to relapse. In this review, we imagine single-cell technologies as the Ariadne's thread that will guide us out of the AML maze, provide a precise identikit of the leukemic cell at single-cell resolution and explore genomic, transcriptomic, epigenetic and proteomic levels.

摘要

急性髓系白血病（AML）是一种由于遗传和表观遗传改变积累而导致的血液肿瘤。患者的预后因AML的遗传异质性而异，这阻碍了治疗的成功。单细胞方法为AML的克隆结构提供了新的见解，揭示了从诊断、治疗到复发的突变历程。在本综述中，我们将单细胞技术想象为引导我们走出AML迷宫的阿里阿德涅之线，以单细胞分辨率提供白血病细胞的精确特征描述，并在基因组、转录组、表观基因组和蛋白质组水平上进行探索。