Shanghai Jiao Tong University School of Medicine, Affiliated Ruijin Hospital, 197 Rui Jin Er Road, Shanghai, 200025, China.
Shanghai Institute of Hematology, 197 Rui Jin Er Road, Shanghai, 200025, China.
Proteomics. 2020 Jul;20(13):e1900228. doi: 10.1002/pmic.201900228. Epub 2020 May 25.
Hematological diseases, including leukemia, lymphoma, and multiple myeloma, are characterized by high heterogeneity with diverse cellular subpopulations. Single-cell RNA sequencing (scRNA-seq), a transformational technology, provides deep insights into cell-to-cell variation in tumor and microenvironment, allows high-resolution dissection of the pathogenic mechanisms of diseases, and affords potential clinical utilities. Recent developments in single-cell transcriptomics and associated technologies and their applications in hematological disorders for unraveling cellular subpopulations, disease pathogenesis, patient stratification, and therapeutic responses are summarized.
血液系统疾病包括白血病、淋巴瘤和多发性骨髓瘤，其特征是具有高度异质性，包含多种细胞亚群。单细胞 RNA 测序（scRNA-seq）是一种变革性技术，可深入了解肿瘤和微环境中的细胞间变异性，能够高分辨率地解析疾病的发病机制，并提供潜在的临床应用价值。本文总结了单细胞转录组学及相关技术的最新进展及其在血液系统疾病中的应用，包括解析细胞亚群、疾病发病机制、患者分层和治疗反应等方面。
