Todeschini Valeria, Anastasia Flavio, Massa Nadia, Marsano Francesco, Cesaro Patrizia, Bona Elisa, Gamalero Elisa, Oddi Ludovica, Lingua Guido
Dipartimento di Scienze ed Innovazione Tecnologica, Università del Piemonte Orientale, 15121 Alessandria, Italy.
Dipartimento per lo Sviluppo Sostenibile e la Transizione Ecologica, Università del Piemonte Orientale, 13100 Vercelli, Italy.
Life (Basel). 2022 Mar 29;12(4):497. doi: 10.3390/life12040497.
L. is a medicinal plant appreciated for the production of artemisinin, a molecule used for malaria treatment. However, the natural concentration of artemisinin is low. Plant nutrition, in particular phosphorus, and arbuscular mycorrhizal (AM) fungi can affect both plant biomass and secondary metabolite production. In this work, plants were ino- culated or not with the AM fungus BEG12 and cultivated for 2 months in controlled conditions at three different phosphatic (P) concentrations (32, 96, and 288 µM). Plant growth parameters, leaf photosynthetic pigment concentrations, artemisinin production, and mineral uptake were evaluated. The different P levels significantly affected the plant shoot growth, AM fungal colonization, and mineral acquisition. High P levels negatively influenced mycorrhizal colonization. The artemisinin concentration was inversely correlated to the P level in the substrate. The fungus mainly affected root growth and nutrient uptake and significantly lowered leaf artemisinin concentration. In conclusion, P nutrition can influence plant biomass production and the lowest phosphate level led to the highest artemisinin concentration, irrespective of the plant mineral uptake. Plant responses to AM fungi can be modulated by cost-benefit ratios of the mutualistic exchange between the partners and soil nutrient availability.
黄花蒿是一种药用植物，因其能产生用于治疗疟疾的青蒿素分子而受到重视。然而，青蒿素的天然浓度较低。植物营养，特别是磷，以及丛枝菌根（AM）真菌，都会影响植物生物量和次生代谢产物的产生。在这项研究中，将植物接种或不接种AM真菌BEG12，并在三种不同的磷酸盐（P）浓度（32、96和288 μM）的可控条件下培养2个月。对植物生长参数、叶片光合色素浓度、青蒿素产量和矿物质吸收进行了评估。不同的磷水平显著影响植物地上部生长、AM真菌定殖和矿物质获取。高磷水平对菌根定殖有负面影响。青蒿素浓度与基质中的磷水平呈负相关。该真菌主要影响根系生长和养分吸收，并显著降低叶片青蒿素浓度。总之，磷营养可以影响植物生物量的产生，无论植物对矿物质的吸收如何，最低的磷酸盐水平都会导致最高的青蒿素浓度。植物对AM真菌的反应可以通过共生伙伴之间互利交换的成本效益比和土壤养分有效性来调节。
