Rapaport F T, Arnold A N, Asari H, Sato K, Miura S, Chanana A, Cronkite E P
Cell Immunol. 1987 Feb;104(2):255-70. doi: 10.1016/0008-8749(87)90028-1.
The removal from stored autologous host bone marrow of a monocytoid cell population by exposure to methylprednisolone is associated with successful introduction of unresponsiveness to renal allografts in irradiated recipients reconstituted with such treated marrow. The eliminated cells are a prominent component of the canine long bone marrow interstitium and share a number of important properties with dendritic cells (DC), including size and shape; poor or nonadherence to plastic or glass surfaces; negative staining for neutral esterase, acid phosphatase, or peroxidase; nonphagocytic; Ia positive, but negative for IgG or IgM; ability to act as accessory cells in augmenting the intensity of allogeneic mixed-lymphocyte reactions. Both cell types are of bone marrow origin and are susceptible to steroids in vitro. The results suggest that the bone marrow interstitial cells identified in the course of this study may be enriched with populations of canine dendritic cell precursors and dendritic cells at various stages of differentiation. The detection of a receptor site for Helix promatia on the surface of such cells may be of usefulness in their further characterization and in the analysis of their precise role in the modulation of allogeneic unresponsiveness.
通过暴露于甲基强的松龙从储存的自体宿主骨髓中去除单核细胞样细胞群体，与用这种处理过的骨髓重建的受辐照受体成功引入对肾同种异体移植的无反应性有关。被清除的细胞是犬长骨髓间质的主要成分，与树突状细胞（DC）具有许多重要特性，包括大小和形状；对塑料或玻璃表面粘附性差或无粘附性；中性酯酶、酸性磷酸酶或过氧化物酶染色阴性；无吞噬作用；Ia阳性，但IgG或IgM阴性；能够作为辅助细胞增强同种异体混合淋巴细胞反应的强度。这两种细胞类型均起源于骨髓，并且在体外对类固醇敏感。结果表明，在本研究过程中鉴定出的骨髓间质细胞可能富含犬树突状细胞前体和处于不同分化阶段的树突状细胞群体。在此类细胞表面检测到螺旋原虫的受体位点可能有助于它们的进一步表征以及分析它们在同种异体无反应性调节中的精确作用。
