Gordon D, Mohai L G, Schwartz S M
Circ Res. 1986 Dec;59(6):633-44. doi: 10.1161/01.res.59.6.633.
Arterial smooth muscle cells become tetraploid with age and hypertension. To further study this phenomenon, neonatal rat aortic smooth muscle cells were placed in cell culture and studied over time. Numerous cells with tetraploid and even octaploid DNA content appeared beginning in primary cultures. These increases in DNA content per cell were determined by quantitative fluorescence microscopy and flow cytometry, and true polyploidy was confirmed by chromosome counts. In contrast, cells from adult rat aortas failed to produce significant polyploid cells over time in culture. In vitro culture of neonatal aortic cells may therefore be a model system for studying the initiation of polyploidy in arterial smooth muscle.
随着年龄增长和患高血压，动脉平滑肌细胞会变成四倍体。为了进一步研究这一现象，将新生大鼠主动脉平滑肌细胞置于细胞培养中并进行长期研究。从原代培养开始就出现了许多具有四倍体甚至八倍体DNA含量的细胞。通过定量荧光显微镜和流式细胞术确定了每个细胞中DNA含量的这些增加，并通过染色体计数证实了真正的多倍体。相比之下，成年大鼠主动脉的细胞在培养过程中未能随着时间产生大量多倍体细胞。因此，新生主动脉细胞的体外培养可能是研究动脉平滑肌多倍体形成起始的一个模型系统。
