Senthilkumaran Chandrika, Kroeker Andrea L, Smith Gregory, Embury-Hyatt Carissa, Collignon Brad, Ramirez-Medina Elizabeth, Azzinaro Paul A, Pickering Bradley S, Diaz-San Segundo Fayna, Weingartl Hana M, de Los Santos Teresa
National Centre for Foreign Animal Disease (NCFAD), Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada.
Public Health Ontario, Santé Publique Ontario, Toronto, ON M5G 1M1, Canada.
Pathogens. 2022 Apr 8;11(4):449. doi: 10.3390/pathogens11040449.
Under experimental conditions, pigs infected with Ebola Virus (EBOV) develop disease and can readily transmit the virus to non-human primates or pigs. In the event of accidental or intentional EBOV infection of domestic pigs, complex and time-consuming safe depopulation and carcass disposal are expected. Delaying or preventing transmission through a reduction in viral shedding is an absolute necessity to limit the spread of the virus. In this study, we tested whether porcine interferon-α or λ3 (porIFNα or porIFNλ3) delivered by a replication-defective human type 5 adenovirus vector (Ad5-porIFNα or Ad5-porIFNλ3) could limit EBOV replication and shedding in domestic pigs. Our results show that pigs pre-treated with Ad5-porIFNα did not develop measurable clinical signs, did not shed virus RNA, and displayed strongly reduced viral RNA load in tissues. A microarray analysis of peripheral blood mononuclear cells indicated that Ad5-porIFNα treatment led to clear upregulation in immune and inflammatory responses probably involved in protection against disease. Our results indicate that administration of Ad5-porIFNα can potentially be used to limit the spread of EBOV in pigs.
在实验条件下，感染埃博拉病毒（EBOV）的猪会发病，并能轻易地将病毒传播给非人类灵长类动物或猪。如果家猪意外或故意感染EBOV，预计将需要进行复杂且耗时的安全扑杀和尸体处理。通过减少病毒脱落来延迟或阻止传播对于限制病毒传播绝对必要。在本研究中，我们测试了由复制缺陷型人5型腺病毒载体（Ad5-porIFNα或Ad5-porIFNλ3）递送的猪干扰素-α或λ3（porIFNα或porIFNλ3）是否能限制家猪体内EBOV的复制和脱落。我们的结果表明，用Ad5-porIFNα预处理的猪没有出现可测量的临床症状，没有排出病毒RNA，并且组织中的病毒RNA载量大幅降低。对外周血单核细胞的微阵列分析表明，Ad5-porIFNα处理导致免疫和炎症反应明显上调，这可能参与了对疾病的保护。我们的结果表明，给予Ad5-porIFNα有可能用于限制EBOV在猪中的传播。
