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微小 RNA 影响人脐静脉内皮细胞的迁移能力。

MicroRNAs Influence the Migratory Ability of Human Umbilical Vein Endothelial Cells.

机构信息

Vascular Surgery, University Clinic Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.

BioQuant, Heidelberg University, 69120 Heidelberg, Germany.

出版信息

Genes (Basel). 2022 Apr 2;13(4):640. doi: 10.3390/genes13040640.

DOI:10.3390/genes13040640
PMID:35456446
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9029696/
Abstract

To identify miRNAs that are involved in cell migration in human umbilical vein endothelial cells (HUVECs), we employed RNA sequencing under high glucose incubation and text mining within the databases miRWalk and TargetScanHuman using 83 genes that regulate HUVECs migration. From both databases, 307 predicted miRNAs were retrieved. Differentially expressed miRNAs were determined by exposing HUVECs to high glucose stimulation, which significantly inhibited the migratory ability of HUVECs as compared to cells cultured in normal glucose. A total of 35 miRNAs were found as differently expressed miRNAs in miRNA sequencing, and 4 miRNAs, namely miR-21-3p, miR-107, miR-143-3p, and miR-106b-5p, were identified as overlapping hits. These were subjected to hub gene analysis and pathway analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG), identifing 71 pathways which were influenced by all four miRNAs. The influence of all four miRNAs on HUVEC migration was phenomorphologically confirmed. miR21 and miR107 promoted migration in HUVECs while miR106b and miR143 inhibited migration. Pathway analysis also revealed eight shared pathways between the four miRNAs. Protein-protein interaction (PPI) network analysis was then performed to predict the functionality of interacting genes or proteins. This revealed six hub genes which could firstly be predicted to be related to HUVEC migration.

摘要

为了鉴定在人脐静脉内皮细胞（HUVEC）中参与细胞迁移的 miRNAs，我们在高糖孵育下进行 RNA 测序，并在 miRWalk 和 TargetScanHuman 数据库中进行文本挖掘，使用 83 个调节 HUVEC 迁移的基因。从这两个数据库中，共检索到 307 个预测的 miRNAs。通过将 HUVEC 暴露于高糖刺激来确定差异表达的 miRNAs，与在正常葡萄糖中培养的细胞相比，这显著抑制了 HUVEC 的迁移能力。在 miRNA 测序中总共发现了 35 个差异表达的 miRNAs，其中 4 个 miRNAs，即 miR-21-3p、miR-107、miR-143-3p 和 miR-106b-5p，被鉴定为重叠命中。对这四个 miRNAs 进行了枢纽基因分析和京都基因与基因组百科全书（KEGG）通路分析，确定了所有四个 miRNAs 都受影响的 71 个通路。所有四个 miRNAs 对 HUVEC 迁移的影响在表型上得到了证实。miR21 和 miR107 促进了 HUVEC 的迁移，而 miR106b 和 miR143 则抑制了迁移。通路分析还揭示了四个 miRNA 之间的八个共享通路。然后进行蛋白质-蛋白质相互作用（PPI）网络分析，以预测相互作用基因或蛋白质的功能。这揭示了六个枢纽基因，它们首先可以预测与 HUVEC 迁移有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac14/9029696/4cbbaac9564d/genes-13-00640-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac14/9029696/142cbb1fcc38/genes-13-00640-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac14/9029696/947c1de03ec5/genes-13-00640-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac14/9029696/f2a9c032aec7/genes-13-00640-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac14/9029696/12523fbe934a/genes-13-00640-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac14/9029696/4cbbaac9564d/genes-13-00640-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac14/9029696/142cbb1fcc38/genes-13-00640-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac14/9029696/83fb88345360/genes-13-00640-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac14/9029696/acfa624726c9/genes-13-00640-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac14/9029696/947c1de03ec5/genes-13-00640-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac14/9029696/f2a9c032aec7/genes-13-00640-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac14/9029696/12523fbe934a/genes-13-00640-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac14/9029696/4cbbaac9564d/genes-13-00640-g008.jpg

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