Department of Biology and Biotechnology 'L. Spallanzani', University of Pavia, via Ferrata 9, 27100 Pavia, Italy.
Genes (Basel). 2022 Apr 9;13(4):667. doi: 10.3390/genes13040667.
SOG1 (Suppressor of the Gamma response 1) is the master-regulator of plant DNA damage response (DDR), a highly coordinated network of DNA damage sensors, transducers, mediators, and effectors, with highly coordinated activities. SOG1 transcription factor belongs to the NAC/NAM protein family, containing the well-conserved NAC domain and five serine-glutamine (SQ) motifs, preferential targets for phosphorylation by ATM and ATR. So far, the information gathered for the SOG1 function comes from studies on the model plant . To expand the knowledge on plant-specific DDR, it is opportune to gather information on other SOG1 orthologues. The current study identified plants where multiple SOG1 homologues are present and evaluated their functions by leveraging the information contained in publicly available transcriptomics databases. This analysis revealed the presence of multiple SOG1 sequences in thirteen plant species, and four (, , , ) were selected for gene expression data mining based on database availability. Additionally, seeds and seedlings exposed to treatments known to activate DDR pathways were used to evaluate the expression profiles of and . The experimental workflow confirmed the data retrieved from transcriptomics datasets, suggesting that the homologues have redundant functions in different plant species.
SOG1（Gamma 反应抑制因子 1）是植物 DNA 损伤应答（DDR）的主要调控因子，是一个高度协调的 DNA 损伤传感器、转导子、介质和效应器网络，具有高度协调的活性。SOG1 转录因子属于 NAC/NAM 蛋白家族，包含高度保守的 NAC 结构域和五个丝氨酸-谷氨酰胺（SQ）基序，是 ATM 和 ATR 磷酸化的优先靶点。到目前为止，SOG1 功能的信息来自于模式植物的研究。为了扩展对植物特异性 DDR 的了解，收集其他 SOG1 同源物的信息是适时的。本研究鉴定了存在多个 SOG1 同源物的植物，并利用公开可用的转录组学数据库中包含的信息来评估它们的功能。该分析表明，在十三种植物物种中存在多个 SOG1 序列，并且基于数据库可用性，选择了四个（,,, ）进行基因表达数据挖掘。此外，利用已知激活 DDR 途径的处理方法对 和 的种子和幼苗进行了处理，以评估它们的表达谱。实验工作流程证实了从转录组数据集检索的数据，表明在不同植物物种中， 同源物具有冗余功能。
