Persad Anil K, Fahmy Hanan A, Anderson Nicholas, Cui Jing, Topalcengiz Zeynal, Jeamsripong Saharuetai, Spanninger Patrick M, Buchanan Robert L, Kniel Kalmia E, Jay-Russell Michele T, Danyluk Michelle D, Rajashekara Gireesh, LeJeune Jeffrey T
Center for Food Animal Health, Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH 44691, USA.
School of Veterinary Medicine, Faculty of Medical Sciences, The University of the West Indies, Eric Williams Medical Sciences Complex, Mt. Hope, Trinidad and Tobago, West Indies.
Microorganisms. 2022 Mar 23;10(4):688. doi: 10.3390/microorganisms10040688.
Subtyping of bacterial isolates of the same genus and species is an important tool in epidemiological investigations. A number of phenotypic and genotypic subtyping methods are available; however, most of these methods are labor-intensive and time-consuming and require considerable operator skill and a wealth of reagents. Matrix-Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF), an alternative to conventional subtyping methods, offers a rapid, reproducible method for bacterial identification with a high sensitivity and specificity and at minimal cost. The purpose of this study was to determine the feasibility of using MALDI-TOF to differentiate between six serovars recovered from experimental microcosms inoculated with known strains of . Following the establishment of a MALDI-TOF reference library for this project, the identity of 843 isolates recovered from these microcosms was assessed using both MALDI-TOF and conventional methods (serotyping/PCR). All 843 isolates were identified as being species. Overall, 803/843 (95%) of these isolates were identified similarly using the two different methods. Positive percent agreement at the serovar level ranged from 79 to 100%, and negative percent agreement for all serovars was greater than 98%. Cohen's kappa ranged from 0.85 to 0.98 for the different serovars. This study demonstrates that MALDI-TOF is a viable alternative for the rapid identification and differentiation of serovars.
对同一属和种的细菌分离株进行亚型分类是流行病学调查中的一项重要工具。有多种表型和基因型亚型分类方法可供使用；然而，这些方法大多 labor-intensive 且耗时，需要相当的操作人员技能和大量试剂。基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）作为传统亚型分类方法的替代方法，提供了一种快速、可重复的细菌鉴定方法，具有高灵敏度和特异性，且成本最低。本研究的目的是确定使用 MALDI-TOF 区分从接种已知菌株的实验微宇宙中回收的六种血清型的可行性。在为该项目建立 MALDI-TOF 参考文库后，使用 MALDI-TOF 和传统方法（血清分型/PCR）对从这些微宇宙中回收的 843 株分离株进行了鉴定。所有 843 株分离株均被鉴定为该种。总体而言，使用两种不同方法对这些分离株中的 803/843（95%）进行了相似的鉴定。血清型水平的阳性百分比一致性范围为 79%至 100%，所有血清型的阴性百分比一致性均大于 98%。不同血清型的 Cohen's kappa 范围为 0.85 至 0.98。本研究表明，MALDI-TOF 是快速鉴定和区分该血清型的可行替代方法。
