鉴定菠菜雌雄花花器官发育相关的 microRNAs。

Identification of Sex Differentiation-Related microRNAs in Spinach Female and Male Flower.

机构信息

College of Life Sciences, Henan Normal University, Xinxiang 453007, China.

出版信息

Int J Mol Sci. 2022 Apr 7;23(8):4090. doi: 10.3390/ijms23084090.

DOI:10.3390/ijms23084090

PMID:35456907

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9029227/

Abstract

Sex determination and differentiation is an important biological process for unisexual flower development. Spinach is a model plant to study the mechanism of sex determination and differentiation of dioecious plant. Till now, little is known about spinach sex determination and differentiation mechanism. MicroRNAs are key factors in flower development. Herein, small RNA sequencing was performed to explore the roles of microRNAs in spinach sex determination and differentiation. As a result, 92 known and 3402 novel microRNAs were identified in 18 spinach female and male flower samples. 74 differentially expressed microRNAs were identified between female and male flowers, including 20 female-biased and 48 male-biased expression microRNAs. Target prediction identified 22 sex-biased microRNA-target pairs, which may be involved in spinach sex determination or differentiation. Among the differentially expressed microRNAs between FNS and M03, 55 microRNAs were found to reside in sex chromosome; one of them, , was functionally studied via genetic transformation. Silencing of resulted in abnormal anther while overexpression of induced early flowering, indicating was a male-promoting factor and validating the reliability of our small RNA sequencing data. Conclusively, this work can supply valuable information for exploring spinach sex determination and differentiation and provide a new insight in studying unisexual flower development.

摘要

性别决定和分化是雌雄异花发育的重要生物学过程。菠菜是研究雌雄异株植物性别决定和分化机制的模式植物。到目前为止，人们对菠菜性别决定和分化的机制知之甚少。miRNAs 是花发育的关键因素。在此，进行了小 RNA 测序，以探讨 miRNAs 在菠菜性别决定和分化中的作用。结果，在 18 个菠菜雌性和雄性花样本中鉴定出 92 个已知和 3402 个新的 miRNAs。在雌性花和雄性花之间鉴定出 74 个差异表达的 microRNAs，包括 20 个雌性偏表达和 48 个雄性偏表达的 microRNAs。靶预测鉴定出 22 个性别偏表达的 microRNA-靶对，它们可能参与菠菜性别决定或分化。在 FNS 和 M03 之间差异表达的 microRNAs 中，有 55 个 microRNAs 位于性染色体上；其中之一，，通过遗传转化进行了功能研究。沉默导致花药异常，而过表达诱导早花，表明是一种雄性促进因子，验证了我们小 RNA 测序数据的可靠性。总之，这项工作可以为探索菠菜性别决定和分化提供有价值的信息，并为研究雌雄异花发育提供新的视角。