CAS Key Laboratory of Topical Plant Resources and Sustainable Use, CAS Center for Excellence in Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, 88 Xuefu Road, Kunming 650223, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Int J Mol Sci. 2022 Apr 18;23(8):4439. doi: 10.3390/ijms23084439.
Nyctinastic leaf movement of Fabaceae is driven by the tiny motor organ pulvinus located at the base of the leaf or leaflet. Despite the increased understanding of the essential role of ()/ () orthologs in determining pulvinus identity in legumes, key regulatory components and molecular mechanisms underlying this movement remain largely unclear. Here, we used WT pulvinus and the equivalent tissue in the mutant to carry out transcriptome and proteome experiments. The omics data indicated that there are multiple cell biological processes altered at the gene expression and protein abundance level during the pulvinus development. In addition, comparative analysis of different leaf tissues provided clues to illuminate the possible common primordium between pulvinus and petiole, as well as the function of ELP1. Furthermore, the auxin pathway, cell wall composition and chloroplast distribution were altered in mutants, verifying their important roles in pulvinus development. This study provides a comprehensive insight into the motor organ of the model legume and further supplies a rich dataset to facilitate the identification of novel players involved in nyctinastic movement.
豆科植物的感夜性叶片运动是由位于叶片或小叶基部的微小运动器官叶枕驱动的。尽管人们越来越了解 ()/ () 同源物在确定豆科植物叶枕身份方面的重要作用，但这种运动的关键调节成分和分子机制在很大程度上仍不清楚。在这里，我们使用 WT 叶枕和 突变体中的等效组织进行了转录组和蛋白质组实验。这些组学数据表明，在叶枕发育过程中，多个细胞生物学过程在基因表达和蛋白质丰度水平上发生了改变。此外，对不同叶片组织的比较分析为阐明叶枕和叶柄之间可能存在的共同原基以及 ELP1 的功能提供了线索。此外，生长素途径、细胞壁组成和叶绿体分布在 突变体中发生改变，验证了它们在叶枕发育中的重要作用。本研究全面深入地了解了模式豆科植物的运动器官，并进一步提供了丰富的数据集，有助于鉴定参与感夜性运动的新参与者。
