College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China.
Viruses. 2022 Apr 15;14(4):825. doi: 10.3390/v14040825.
Influenza A virus (IAV) is an important zoonotic pathogen, posing a severe burden for the health of both animals and humans. Many host factors are involved in the life cycle of IAV to regulate its replication. Herein, we identified sorting nexin-16 (SNX16) as a new host factor that negatively modulates the replication of IAV. When transiently overexpressed in cells, SNX16 appears to be expressed as two obvious bands. Mutagenesis analysis indicated that the amino acid residue R144 of SNX16 was responsible for its two-band expression phenotype. We found that the R144A mutation of SNX16 changed its cellular distribution in A549 cells and partially weakened the inhibitory effect of SNX16 on IAV replication. Further investigation revealed that SNX16 could negatively regulate the early stage of the replication cycle of IAV. Taken together, our results demonstrated that SNX16 is a novel restriction host factor for the replication of IAV by engaging in the early stage of IAV life cycle, and a single amino acid residue at position 144 plays an important role in the cellular distribution and anti-influenza function of SNX16.
甲型流感病毒（IAV）是一种重要的人畜共患病病原体，对动物和人类的健康都构成了严重威胁。许多宿主因素参与了 IAV 的生命周期，以调节其复制。在此，我们鉴定出分选连接蛋白-16（SNX16）是一种新的宿主因子，可负调控 IAV 的复制。当在细胞中转染瞬时过表达时，SNX16 似乎表达为两个明显的条带。突变分析表明，SNX16 的氨基酸残基 R144 负责其双带表达表型。我们发现 SNX16 的 R144A 突变改变了其在 A549 细胞中的细胞分布，并部分减弱了 SNX16 对 IAV 复制的抑制作用。进一步的研究表明，SNX16 可以负调控 IAV 复制周期的早期阶段。综上所述，我们的研究结果表明，SNX16 通过参与 IAV 生命周期的早期阶段，成为 IAV 复制的一种新型限制宿主因子，位于位置 144 的单个氨基酸残基在 SNX16 的细胞分布和抗流感功能中发挥重要作用。
