Department of Chemistry, Yonsei University, Seoul 03722, Korea.
Sensors (Basel). 2022 Apr 15;22(8):3064. doi: 10.3390/s22083064.
A highly sensitive tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy)) electrogenerated chemiluminescence (ECL) sensor based on a graphene-titania-Nafion composite film has been prepared in a simple one-step manner. In the present work, a highly concentrated 0.1 M Ru(bpy) solution was mixed with an as-prepared graphene-titania-Nafion composite solution (1:20, /), and then a small aliquot (2 µL) of the resulting mixture solution was cast on a glassy carbon electrode surface. This one-step process for the construction of an ECL sensor shortens the fabrication time and leads to reproducible ECL signals. Due to the synergistic effect of conductive graphene and mesoporous sol-gel derived titania-Nafion composite, the present ECL sensor leads to a highly sensitive detection of tripropylamine from 1.0 × 10 M to 2.0 × 10 M with a detection limit of 0.8 nM (S/N = 3), which is lower in comparison to that of the ECL sensor based on the corresponding ECL sensor based on the titania-Nafion composite containing carbon nanotube. The present ECL sensor also shows a good response for nicotinamide adenine dinucleotide hydrogen (NADH) from 1.0 × 10 M to 1.0 × 10 M with a detection limit of 0.4 µM (S/N = 3). Thus, the present ECL sensor can offer potential benefits in the development of dehydrogenase-based biosensors.
一种基于石墨烯-二氧化钛-纳滤复合膜的高灵敏三（2,2'-联吡啶）钌（Ru(bpy))电致化学发光（ECL）传感器已被制备。在本工作中，将高浓度的 0.1 M Ru(bpy)溶液与预先制备的石墨烯-二氧化钛-纳滤复合溶液（1:20，/）混合，然后将所得混合溶液的一小部分（2 µL）滴涂在玻碳电极表面。这种一步法制备 ECL 传感器缩短了制备时间并得到重现性良好的 ECL 信号。由于导电石墨烯和介孔溶胶-凝胶衍生的 TiO2-Nafion 复合材料的协同作用，本 ECL 传感器对三丙胺的检测具有高灵敏度，从 1.0 × 10 M 到 2.0 × 10 M，检测限为 0.8 nM（S/N = 3），比基于相应的 TiO2-Nafion 复合材料含碳纳米管的 ECL 传感器更低。本 ECL 传感器对烟酰胺腺嘌呤二核苷酸氢（NADH）也有良好的响应，从 1.0 × 10 M 到 1.0 × 10 M，检测限为 0.4 µM（S/N = 3）。因此，本 ECL 传感器可为基于脱氢酶的生物传感器的发展提供潜在的益处。
