Liang Ching-Chung, Huang Wen-Chu, Shaw Steven W, Huang Yung-Hsin, Lee Tsong-Hai
Female Urology Section, Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital Linkou Medical Center, Taoyuan, Taiwan.
College of Medicine, Chang Gung University, Taoyuan, Taiwan.
Sci Rep. 2022 Apr 23;12(1):6679. doi: 10.1038/s41598-022-10640-y.
The present study examined whether bladder detrusor dysfunction due to partial bladder outlet obstruction (pBOO) could be improved after the treatment of human amniotic fluid stem cells (hAFSCs). 72 female rats were grouped into sham operation, pBOO, and pBOO with hAFSCs treatment (pBOO + hAFSCs) for in vitro and in vivo studies. Bladder weight, bladder wall thickness, the ratio of collagen to smooth muscle and the levels of positive CD11b/c and HIS48 cells was significantly increased after pBOO but improved after hAFSCs treatment. Cystometries showed impaired bladder function after pBOO. Protein and mRNA levels of hypoxia inducible factor-1α, CCL2, interleukin-1β, transforming growth factor-β1 (TGF-β1), connective tissue growth factor (CTGF), α-smooth muscle actin, collagen I and collagen III were increased at 2 and/or 6 weeks, but proteins and mRNA expressions of protein gene product 9.5 were decreased at 2 and 6 weeks after pBOO. These abnormalities were improved after hAFSCs treatment. The expressions of TGF-β1 and CTGF in cultured detrusor cells of pBOO rats were increased but were improved after hAFSCs treatment. The present results showed hAFSCs treatment could improve bladder detrusor dysfunction in pBOO rats, which may be related to the reduction of inflammatory and pro-fibrotic markers in detrusor muscle cells.
本研究检测了部分膀胱出口梗阻（pBOO）所致膀胱逼尿肌功能障碍在人羊水干细胞（hAFSCs）治疗后是否能够得到改善。将72只雌性大鼠分为假手术组、pBOO组和hAFSCs治疗的pBOO组（pBOO + hAFSCs），用于体外和体内研究。pBOO后膀胱重量、膀胱壁厚度、胶原蛋白与平滑肌的比例以及CD11b/c阳性细胞和HIS48细胞水平显著增加，但hAFSCs治疗后有所改善。膀胱测压显示pBOO后膀胱功能受损。缺氧诱导因子-1α、CCL2、白细胞介素-1β、转化生长因子-β1（TGF-β1）、结缔组织生长因子（CTGF）、α-平滑肌肌动蛋白、胶原蛋白I和胶原蛋白III的蛋白质和mRNA水平在2周和/或6周时升高，但pBOO后2周和6周时蛋白基因产物9.5的蛋白质和mRNA表达降低。hAFSCs治疗后这些异常情况得到改善。pBOO大鼠培养的逼尿肌细胞中TGF-β1和CTGF的表达增加，但hAFSCs治疗后有所改善。目前的结果表明，hAFSCs治疗可改善pBOO大鼠的膀胱逼尿肌功能障碍，这可能与逼尿肌细胞中炎症和促纤维化标志物的减少有关。
