Vishteh A, Thomas I, Imamura T
Immunopharmacology. 1986 Dec;12(3):187-92. doi: 10.1016/0162-3109(86)90002-0.
The effect of eugenol on selected parameters of the immune response of C57BL/6 mice was studied. In a dose-response study, eugenol at high doses completely inhibited the plaque-forming cell responses of splenocytes to the T-dependent antigen (sheep erythrocytes) both in vitro and in vivo. In vitro, at concentrations higher than 1.0 mM, eugenol was found to be cytotoxic. In vivo, however, at very low doses there appeared to be a suppression of the plaque-forming cell response to sheep erythrocytes, reaching maximal suppression at 0.1 mumol eugenol/kg mouse body weight. This was followed by enhancement of the response, peaking at 0.25 mumol eugenol/kg mouse body weight. These changes correlated fairly well with body weight changes. Thymic weights appeared to remain unchanged for all doses except the 1.0 mumol eugenol/kg body weight, which was significantly (p less than 0.05) higher than the rest of the groups. Natural killer activity at all effector:target ratios (100:1, 50:1, 25:1) was significantly (p less than 0.05) enhanced at both 0.25 and 2.5 mumol eugenol/kg body weight. Overall, eugenol seems to have dose-dependent suppressive and enhancing effects on the immune response. These effects represent an atypical multiphasic response in which an inversed dose-response relationship is observed.
研究了丁香酚对C57BL/6小鼠免疫反应所选参数的影响。在剂量反应研究中，高剂量丁香酚在体外和体内均完全抑制了脾细胞对T细胞依赖性抗原（绵羊红细胞）的空斑形成细胞反应。在体外，当浓度高于1.0 mM时，发现丁香酚具有细胞毒性。然而，在体内，极低剂量时似乎对绵羊红细胞的空斑形成细胞反应有抑制作用，在丁香酚0.1 μmol/kg小鼠体重时达到最大抑制。随后反应增强，在丁香酚0.25 μmol/kg小鼠体重时达到峰值。这些变化与体重变化相当吻合。除了丁香酚1.0 μmol/kg体重组外，所有剂量组的胸腺重量似乎保持不变，该组显著高于其他组（p<0.05）。在丁香酚0.25和2.5 μmol/kg体重时，所有效应细胞：靶细胞比例（100:1、50:1、25:1）下的自然杀伤活性均显著增强（p<0.05）。总体而言，丁香酚似乎对免疫反应具有剂量依赖性的抑制和增强作用。这些效应代表了一种非典型的多相反应，其中观察到了反向剂量反应关系。
