探究工业规模下膜覆盖好氧堆肥中减少奶牛粪便甲烷生成的微生物机制。
Exploring the microbial mechanism of reducing methanogenesis during dairy manure membrane-covered aerobic composting at industrial scale.
机构信息
Engineering Laboratory for AgroBiomass Recycling & Valorizing, College of Engineering, China Agricultural University, Beijing 100083, China.
Engineering Laboratory for AgroBiomass Recycling & Valorizing, College of Engineering, China Agricultural University, Beijing 100083, China.
出版信息
Bioresour Technol. 2022 Jun;354:127214. doi: 10.1016/j.biortech.2022.127214. Epub 2022 Apr 21.
In this study, the microbial mechanism of reducing methanogenesis during membrane-covered aerobic composting from solid dairy manure was investigated. An industrial-scale experiment was carried out to compare a static composting group (SC) and a forced aeration composting group (AC) with a semipermeable membrane-covered composting group (MC + AC). The results showed that the semipermeable membrane-covered could improve the oxygen utilization rate and inhibit the anaerobic bacterial genus Hydrogenispora and archaea order Methanobacteriales. During the membrane-covered period, the acetoclastic methanogenesis module in MC + AC, AC and SC decreased by 0.58%, 0.05% and 0.04%, respectively, and the cdhC gene in the acetoclastic pathway was found to be decreased by 65.51% only in MC + AC. Changes in methane metabolism pathways resulted in a 27.48% lower average methane concentration in MC + AC than in SC. Therefore, the semipermeable membrane-covered strategy can effectively reduce methane production during dairy manure aerobic composting by restricting the methanogenesis of the acetoclastic pathway.
本研究旨在探讨覆盖膜好氧堆肥过程中减少甲烷生成的微生物机制，利用工业规模的实验，对比静态堆肥组（SC）、强制通风堆肥组（AC）和半透膜覆盖强制通风堆肥组（MC+AC）。结果表明，半透膜覆盖可以提高氧气利用率，抑制产氢菌属和甲烷杆菌目等厌氧细菌属。在覆盖膜期间，MC+AC、AC 和 SC 中的乙酸营养型产甲烷模块分别降低了 0.58%、0.05%和 0.04%，并且仅在 MC+AC 中发现乙酸途径中的 cdhC 基因降低了 65.51%。甲烷代谢途径的变化导致 MC+AC 中的平均甲烷浓度比 SC 低 27.48%。因此，半透膜覆盖策略可以通过限制乙酸营养型途径的产甲烷作用，有效减少奶牛粪便好氧堆肥过程中的甲烷生成。
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