Manis Cristina, Malegori Cristina, Alladio Eugenio, Vincenti Marco, Garofano Paolo, Barni Filippo, Berti Andrea, Oliveri Paolo
Dipartimento di Chimica, Università degli Studi di Torino, Torino, Italy.
Dipartimento di Farmacia (DIFAR), Università degli Studi di Genova, Genova, Italy.
Talanta. 2022 Aug 1;245:123472. doi: 10.1016/j.talanta.2022.123472. Epub 2022 Apr 12.
From a criminalistic point of view, the accurate dating of biological traces found at the crime scene, together with its compatibility with the estimated crime perpetration timeframe, enables to limit the number of suspects by assessing their alibis and clarifying the sequence of events. The present study delineates, for the first time, the possibility of dating biological fluids such as semen and urine, as well as blood traces, by using a novel non-destructive analytical strategy based on hyperspectral imaging in the near infared region (HSI-NIR), coupled with multivariate regression methods. Investigated aspects of the present study include not only the progressive degradation of the biological trace itself, but also the effects of its interactions with the support on which it is absorbed, in particular the hydrophilic vs. hydrophobic character of fabric tissues. Results are critically discussed, highlighting potential and limitations of the proposed approach for a practical implementation.
从刑事鉴定的角度来看，对犯罪现场发现的生物痕迹进行准确的年代测定，以及其与估计的犯罪实施时间范围的兼容性，能够通过评估嫌疑人的不在场证明并厘清事件顺序来减少嫌疑人数量。本研究首次描述了通过一种基于近红外区域高光谱成像（HSI-NIR）的新型无损分析策略，并结合多元回归方法，对精液、尿液等生物液体以及血迹进行年代测定的可能性。本研究调查的方面不仅包括生物痕迹本身的渐进性降解，还包括其与吸收它的载体相互作用的影响，特别是织物组织的亲水性与疏水性特征。对结果进行了批判性讨论，强调了所提出方法在实际应用中的潜力和局限性。
