Pua Aileen, Goh Rui Min Vivian, Huang Yunle, Tang Vivien Chia Yen, Ee Kim-Huey, Cornuz Maurin, Liu Shao Quan, Lassabliere Benjamin, Yu Bin
Mane SEA Pte Ltd, 3 Biopolis Drive, #07-17/18/19 Synapse, Singapore 138623, Sigapore; Department of Food Science and Technology, National University of Singapore, S14 Level 5, Science Drive 2, Singapore 117542, Sigapore.
Mane SEA Pte Ltd, 3 Biopolis Drive, #07-17/18/19 Synapse, Singapore 138623, Sigapore.
Food Chem. 2022 Sep 15;388:132971. doi: 10.1016/j.foodchem.2022.132971. Epub 2022 Apr 13.
Coffee has attracted significant research interest owing to its complex volatile composition and aroma, which imparts a pleasant sensorial experience that remains challenging to analyse and interpret. This review summarises analytical challenges associated with coffee's volatile and matrix complexity, and recent developments in instrumental techniques to resolve them. The benefits of state-of-the-art analytical techniques applied to coffee volatile analysis from experimental design to sample preparation, separation, detection, and data analysis are evaluated. Complementary method selection coupled with progressive experimental design and data analysis are vital to unravel the increasing comprehensiveness of coffee volatile datasets. Considering this, analytical workflows for conventional, targeted, and untargeted coffee volatile analyses are thus proposed considering the trends towards sorptive extraction, multidimensional gas chromatography, and high-resolution mass spectrometry. In conclusion, no single analytical method addresses coffee's complexity in its entirely, and volatile analysis must be tailored to the key objectives and concerns of the analyst.
由于咖啡复杂的挥发性成分和香气，它吸引了大量的研究兴趣，这些挥发性成分和香气带来了令人愉悦的感官体验，但对其进行分析和解释仍具有挑战性。本综述总结了与咖啡挥发性和基质复杂性相关的分析挑战，以及解决这些挑战的仪器技术的最新进展。评估了从实验设计到样品制备、分离、检测和数据分析等应用于咖啡挥发性分析的先进分析技术的优势。互补方法的选择与渐进式实验设计和数据分析相结合，对于揭示咖啡挥发性数据集日益增长的全面性至关重要。考虑到这一点，结合吸附萃取、多维气相色谱和高分辨率质谱的发展趋势，提出了传统、靶向和非靶向咖啡挥发性分析的分析工作流程。总之，没有一种单一的分析方法能够完全解决咖啡的复杂性，挥发性分析必须根据分析师的关键目标和关注点进行定制。
