从未经诱导的破伤风梭菌培养物中分离类噬菌体颗粒。

Isolation of bacteriophage-like particles from uninduced Clostridium tetani cultures.

作者信息

Brown K J, Brown P A

出版信息

Aust J Exp Biol Med Sci. 1978 Apr;56(2):139-45. doi: 10.1038/icb.1978.15.

DOI:10.1038/icb.1978.15

Abstract

The isolation of hexagonal-headed, tailless, bacteriophage-like particles from uninduced cultures of Clostridium tetani is described. Clear, round, 1--3 mm diameter plaques were noted on Clostrisel agar plates, which were overlaid with soft agar inoculated with 7--14 day broth cultures. Particles were detected by transmission electron microscopy from broth cultures seeded with scrapings from the plaques. Both electron dense and electron lucent heads were noted. An electron dense head was observed attached to the surface of a dividing bacterium.

摘要

本文描述了从破伤风梭菌未诱导培养物中分离出六头、无尾、噬菌体样颗粒的过程。在接种了7 - 14天肉汤培养物的软琼脂覆盖的Clostrisel琼脂平板上，发现了直径为1 - 3毫米的清晰圆形噬菌斑。通过透射电子显微镜从接种了噬菌斑刮取物的肉汤培养物中检测到颗粒。观察到电子致密和电子透亮的头部。在一个正在分裂的细菌表面观察到一个电子致密的头部。