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酰基载体蛋白的纯化及核磁共振表征

Purification and NMR characterization of acyl carrier protein.

作者信息

Holak T A, Frederick A F, Prestegard J H

出版信息

J Biol Chem. 1987 Mar 15;262(8):3685-9.

PMID:3546316
Abstract

The acyl carrier protein preparation obtained using the 2-propanol method of Rock and Cronan (Rock, C. O., and Cronan, J. E., Jr. (1981) Methods Enzymol. 71, 341-351) can be further purified with reversed-phase high-performance liquid chromatography. A homogeneous sample of acyl carrier protein is obtained as determined by NMR and reversed-phase high-performance liquid chromatography.

摘要

采用Rock和Cronan的异丙醇法（Rock, C. O., and Cronan, J. E., Jr. (1981) Methods Enzymol. 71, 341 - 351）制备的酰基载体蛋白制剂可用反相高效液相色谱进一步纯化。通过核磁共振和反相高效液相色谱测定，获得了酰基载体蛋白的均一样品。

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