Santos Carmen, Martins Davide Coelho, González-Bernal María José, Rubiales Diego, Vaz Patto Maria Carlota
Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.
Institute for Sustainable Agriculture, Consejo Superior de Investigaciones Científicas, Córdoba, Spain.
Front Plant Sci. 2022 Apr 7;13:837613. doi: 10.3389/fpls.2022.837613. eCollection 2022.
Rusts are among the most important foliar biotrophic fungal diseases in legumes. crop can be severely damaged by , to which partial resistance has been identified. Nevertheless, the underlying genetic basis and molecular mechanisms of this resistance are poorly understood in . To prioritise the causative variants controlling partial resistance to rust in , a recombinant inbred line (RIL) population, segregating for response to this pathogen, was used to combine the detection of related phenotypic- and expression-quantitative trait loci (pQTLs and eQTLs, respectively). RILs' disease severity (DS) was recorded in three independent screenings at seedling (growth chamber) and in one season of exploratory screening at adult plant stage (semi-controlled field conditions). A continuous DS range was observed in both conditions and used for pQTL mapping. Different pQTLs were identified under the growth chamber and semi-controlled field conditions, indicating a distinct genetic basis depending on the plant developmental stage and/or the environment. Additionally, the expression of nine genes related to resistance in was quantified for each RIL individual and used for eQTL mapping. One -eQTL and one trans-eQTL were identified controlling the expression variation of one gene related to rust resistance - a member of glycosyl hydrolase family 17. Integrating phenotyping, gene expression and linkage mapping allowed prioritising four candidate genes relevant for disease-resistance precision breeding involved in adaptation to biotic stress, cellular, and organelle homeostasis, and proteins directly involved in plant defence.
锈病是豆科植物中最重要的叶部活体营养型真菌病害之一。该病害会对作物造成严重损害，不过已鉴定出部分抗性。然而，在[具体植物名称]中，这种抗性的潜在遗传基础和分子机制仍知之甚少。为了确定控制[具体植物名称]对锈病部分抗性的致病变异，利用一个对该病原体反应进行分离的重组自交系（RIL）群体，分别结合相关表型和表达数量性状位点（分别为pQTL和eQTL）的检测。在幼苗期（生长室）的三次独立筛选以及成株期（半控制田间条件）的一个季节探索性筛选中记录了RILs的病害严重程度（DS）。在两种条件下均观察到连续的DS范围，并用于pQTL定位。在生长室和半控制田间条件下鉴定出了不同的pQTL，表明其遗传基础因植物发育阶段和/或环境而异。此外，对每个RIL个体定量了[具体植物名称]中九个与锈病抗性相关基因的表达，并用于eQTL定位。鉴定出一个顺式-eQTL和一个反式-eQTL，控制着一个与锈病抗性相关基因（糖基水解酶家族17的一个成员）的表达变异。整合表型分析、基因表达和连锁图谱，能够确定四个与抗病精准育种相关的候选基因，这些基因参与了对生物胁迫的适应、细胞和细胞器稳态，以及直接参与植物防御的蛋白质。
