记录和定量收缩心肌细胞内 pH 值的方案。

Department of Internal Medicine, University of California, Davis, Davis, CA 95616, USA.

Department of Physiology and Cell Biology, University of Nevada, Reno School of Medicine, Reno, NV 89557, USA.

STAR Protoc. 2022 Apr 12;3(2):101301. doi: 10.1016/j.xpro.2022.101301. eCollection 2022 Jun 17.

Intracellular pH (pH) plays critical roles in the regulation of cardiac function. Methods and techniques for cardiac pH measurement have continued to evolve since early 1960s. Fluorescent microscopy is the most recently developed technique with several advantages over other techniques including higher spatial and temporal resolutions, and feasibility for contracting cell measurement. Here, we describe detailed methods for mouse cardiomyocyte isolation, and simultaneous measurement and quantification of pH and sarcomere length in contracting cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Lyu et al. (2022).

细胞内 pH（pH）在心脏功能调节中起着关键作用。自 20 世纪 60 年代初以来，心脏 pH 测量的方法和技术不断发展。荧光显微镜是最近发展起来的技术，与其他技术相比具有几个优势，包括更高的空间和时间分辨率，以及用于收缩细胞测量的可行性。在这里，我们描述了用于分离小鼠心肌细胞的详细方法，以及在收缩的心肌细胞中同时测量和定量 pH 和肌节长度的方法。有关该方案使用和执行的完整详细信息，请参阅 Lyu 等人。（2022 年）。