使用小鼠 4 细胞胚胎的核转移系统，直接从同种和异种核中诱导胚胎转录本。

Nuclear transfer system for the direct induction of embryonic transcripts from intra- and cross-species nuclei using mouse 4-cell embryos.

机构信息

Laboratory of Molecular Developmental Biology, Faculty of Biology-Oriented Science and Technology, Kindai University, Wakayama 649-6493, Japan.

Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Site, CB2 3EG Cambridge, UK.

出版信息

STAR Protoc. 2022 Apr 11;3(2):101284. doi: 10.1016/j.xpro.2022.101284. eCollection 2022 Jun 17.

DOI:10.1016/j.xpro.2022.101284

PMID:35463476

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9019708/

Abstract

Reprogramming of somatic nuclei toward the embryonic state has been studied using nuclear transfer (NT) to an oocyte at the metaphase II (MII) stage. In this NT, a somatic nucleus transplanted into an MII oocyte of the same species undergoes DNA replication and cell division before activating embryonic genes. Here, we describe a direct NT protocol using 4-cell stage mouse embryos that enables reprogramming of intra- and cross-species nuclei to express embryonic genes without requiring DNA replication and cell division. For complete details on the use and execution of this protocol, please refer to Tomikawa et al. (2021).

摘要

体细胞细胞核重编程为胚胎状态的研究已经使用核移植（NT）到中期 II 期（MII）卵母细胞进行了研究。在这种 NT 中，移植到同种 MII 卵母细胞中的体细胞核在激活胚胎基因之前经历 DNA 复制和细胞分裂。在这里，我们描述了一种使用 4 细胞期小鼠胚胎的直接 NT 方案，该方案能够对同种和异种细胞核进行重编程，以表达胚胎基因，而无需进行 DNA 复制和细胞分裂。有关此协议的使用和执行的完整详细信息，请参阅 Tomikawa 等人。（2021 年）。