天然产物的化学表征与抗氧化剂筛选：以刺宁棘为例。
Chemical profiling and antioxidants screening from natural products: using CiNingJi as an example.
作者信息
Zhang Yida, Zheng Peiyan, Yan Guanyu, Zhuo Yue, Wu Jian-Lin, Sun Baoqing
机构信息
Present Address: Department of Allergy and Clinical Immunology, National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease, State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, No.151 Yanjiang Xi Lu, Yuexiu District, Guangzhou, China.
Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, 510405 Guangzhou, China.
出版信息
Food Sci Biotechnol. 2022 Mar 12;31(4):407-421. doi: 10.1007/s10068-022-01049-4. eCollection 2022 Apr.
UNLABELLED
Natural products with good antioxidative properties have been paid increased attention globally. However, due to its chemical complexity, it is difficult to find out its antioxidative compounds. Herein, the chemical profiling and antioxidant capacity of CiNingJi (CNJ) were analyzed, as an example. By using UHPLC-Q-TOF/MS, a total of 82 compounds were tentatively deduced. Furthermore, its free radical scavenging capacity was assessed by different spectrophotometric-based assays. The result showed that one ingredient, , plays a critical role in its antioxidant activity. In addition, 18 potential antioxidants were screened out in CNJ by comparing the difference of it with and without DPPH reaction. They were identified mainly as catechin, ellagic acid, kajiichigoside F1, and their derivatives or isomers. With the further quantification of major found antioxidants, our results may provide some knowledge on predicting the antioxidative compounds of natural products.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s10068-022-01049-4.
未标注
具有良好抗氧化性能的天然产物在全球范围内受到越来越多的关注。然而，由于其化学复杂性，很难找出其抗氧化化合物。在此，以刺柠吉（CNJ）为例，分析其化学图谱和抗氧化能力。通过超高效液相色谱-四极杆飞行时间质谱联用仪（UHPLC-Q-TOF/MS），初步推断出总共82种化合物。此外，通过不同的基于分光光度法的测定评估其自由基清除能力。结果表明，一种成分在其抗氧化活性中起关键作用。此外，通过比较刺柠吉在有和没有二苯基苦味酰基自由基（DPPH）反应时的差异，筛选出18种潜在的抗氧化剂。它们主要被鉴定为儿茶素、鞣花酸、梾木苷F1及其衍生物或异构体。随着对主要发现的抗氧化剂的进一步定量分析，我们的结果可能为预测天然产物的抗氧化化合物提供一些知识。
补充信息
在线版本包含可在10.1007/s10068-022-01049-4获取的补充材料。
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