口服 S2-Ag85DNA 疫苗激活肠道细胞 dsDNA 和 RNA 传感器促进肠道抗原呈递。
Oral S2-Ag85 DNA Vaccine Activated Intestinal Cell dsDNA and RNA Sensors to Promote the Presentation of Intestinal Antigen.
机构信息
Innovative Institute of Zoonoses, Inner Mongolia Minzu University, Tongliao 028000, China.
Brucellosis Prevention and Treatment Engineering Research Center of Inner Mongolia Autonomous Region, Tongliao 028000, China.
出版信息
J Immunol Res. 2022 Apr 13;2022:7200379. doi: 10.1155/2022/7200379. eCollection 2022.
OBJECTIVE
To explore the molecular mechanism by which oral S2-Ag85DNA vaccines present intestinal antigens. The oral S2-Ag85 vaccine has been shown to protect the human body and effectively improve the titration of the vaccine by acting on intestinal mucosa cells and enhancing their immunogenicity.
METHOD
Mice were immunized with the recombinant S2-Ag85 vaccine, and antibody secretion was then detected in the intestinal tissue. The molecular mechanisms of detection sensor molecules RIG-1, Pol III, and related conductor transductor molecules DAI, STING, AIM2, IRF3, and IRF7 were determined by separating intestinal IEC, DC, and IELC cells.
RESULTS
The S2-Ag85A vaccine was effective in activating dsDNA and RNA transduction pathways in intestinal cells and improving intestinal antigen presentation in mice.
目的
探索口服 S2-Ag85DNA 疫苗呈现肠道抗原的分子机制。口服 S2-Ag85 疫苗已被证明通过作用于肠黏膜细胞并增强其免疫原性来保护人体并有效提高疫苗效价。
方法
用重组 S2-Ag85 疫苗免疫小鼠，然后检测肠道组织中的抗体分泌。通过分离肠 IEC、DC 和 IELC 细胞，确定检测传感器分子 RIG-1、Pol III 和相关导体转导分子 DAI、STING、AIM2、IRF3 和 IRF7 的分子机制。
结果
S2-Ag85A 疫苗可有效激活肠细胞中的 dsDNA 和 RNA 转导途径，提高小鼠肠道抗原呈递。
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