Department of Cell and Molecular Biology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India.
Department of Oral Pathology, SGT Dental College Hospital & Research Institute, Gurugram, India.
Oral Dis. 2023 Jul;29(5):1894-1904. doi: 10.1111/odi.14219. Epub 2022 May 3.
The objective of the study is to understand the role of experimentally validated microRNAs (miRNAs) contributing to the acquisition of oncogenic phenotype in oral submucous fibrosis (OSF) by computational analysis. A comprehensive review was carried out to corroborate and summarize altered miRNA expression in OSF by retrieving relevant publications querying MEDLINE, Web of Science, Embase, and Scopus. The association between the miRNA-mRNA was performed using miRTarBase 8.0. The visualization of the miRNA-mRNA interaction was plotted using Cytoscape. MIENTURNET was used for the pathway analysis. Enrichment analysis was carried out for elucidating the hierarchical functions of miRNAs related to the acquisition of biological processes involved in the development of cancer. Thirteen miRNAs (hsa-miR-499a, hsa-miR-200b, hsa-miR-200c, hsa-miR-1246, hsa-miR-31, hsa-miR-10b, hsa-miR-21, hsa-miR-203, hsa-miR-455, hsa-miR-760, hsa-miR-623, hsa-miR-610, and hsa-miR-509-3-5p) were found to be deregulated in OSF. A total of 371 experimentally validated genes were shown to be interacting with the OSF-associated miRNAs. The targets of antifibrotic and profibrotic miRNAs were enriched in the cancer-related pathways. Dysregulated miRNA and its target genes illustrate the physiological role of miRNAs in fibrosis. Understanding the miRNA-mediated fibrotic signaling and targetting the specific miRNA-target gene interaction might provide relevant cues to ameliorate the fibrotic disease.
本研究旨在通过计算分析，了解经实验验证的 microRNAs（miRNAs）在口腔黏膜下纤维化（OSF）获得致癌表型中的作用。通过检索 MEDLINE、Web of Science、Embase 和 Scopus 中相关的出版物，进行了全面的综述，以证实和总结 OSF 中 miRNA 表达的改变。使用 miRTarBase 8.0 进行 miRNA-mRNA 关联。使用 Cytoscape 绘制 miRNA-mRNA 相互作用的可视化图。使用 MIENTURNET 进行通路分析。进行富集分析，以阐明与获得涉及癌症发展的生物学过程相关的 miRNA 的分层功能。发现 13 个 miRNA（hsa-miR-499a、hsa-miR-200b、hsa-miR-200c、hsa-miR-1246、hsa-miR-31、hsa-miR-10b、hsa-miR-21、hsa-miR-203、hsa-miR-455、hsa-miR-760、hsa-miR-623、hsa-miR-610 和 hsa-miR-509-3-5p）在 OSF 中失调。共显示 371 个经实验验证的基因与 OSF 相关的 miRNAs 相互作用。抗纤维化和促纤维化 miRNA 的靶基因富集在癌症相关通路中。失调的 miRNA 及其靶基因说明了 miRNA 在纤维化中的生理作用。了解 miRNA 介导的纤维化信号和靶向特定的 miRNA-靶基因相互作用可能为改善纤维化疾病提供相关线索。
