KU Leuven Kulak, Research Unit Food & Lipids, E. Sabbelaan, 8500 Kortrijk, Belgium.
Leuven Food Science and Nutrition Research Centre (LFoRCe), KU Leuven, Kasteelpark Arenberg 20, 3001 Leuven, Belgium.
J Agric Food Chem. 2022 May 11;70(18):5691-5700. doi: 10.1021/acs.jafc.1c07915. Epub 2022 Apr 26.
Several species of microalgae are promising as an alternative source of omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA). Photoautotrophic species show the greatest potential, since incorporating them into food products leads to oxidatively stable products; however, the presence of photosensitizers could reduce the shelf-life due to the appearance of photo-oxidation on exposure to light. This study investigated the oxidative impact of illumination for aqueous model suspensions enriched with (phototrophic microalgae─containing potential photosensitizers) and (heterotrophic microalgae─lacking photosensitizers) during storage for 28 days at 37 °C. Primary (peroxide value) and secondary (volatiles with gas chromatography (GC)-mass spectrometry) oxidation products, n-3 LC-PUFA content (GC), and pigments (high-pressure liquid chromatography) were assessed. The results showed that photo-oxidation did not cause oxidative instability for samples compared with strong autoxidation in samples. For the enriched suspensions, only minimal photo-oxidation could be detected and the n-3 LC-PUFA content remained stable throughout storage regardless of illumination.
几种微藻有望成为ω-3 长链多不饱和脂肪酸（n-3 LC-PUFA）的替代来源。自养物种具有最大的潜力，因为将它们纳入食品产品会导致氧化稳定的产品；然而，由于在光照下出现光氧化，存在光敏剂可能会降低保质期。本研究在 37°C 下储存 28 天时，调查了富含 （含有潜在光敏剂的光养微藻）和 （缺乏光敏剂的异养微藻）的水相模型悬浮液的光照对氧化的影响。评估了初级（过氧化物值）和次级（气相色谱（GC）-质谱联用仪测定的挥发性）氧化产物、n-3 LC-PUFA 含量（GC）和色素（高压液相色谱）。结果表明，与 样品的强烈自氧化相比，光照不会导致 样品发生氧化不稳定性。对于富集中的悬浮液，仅能检测到最小程度的光氧化，并且 n-3 LC-PUFA 含量在整个储存过程中保持稳定，无论是否有光照。
