Université de Strasbourg, CNRS, IPHC UMR7178, Strasbourg, France; Infrastructure Nationale de Protéomique ProFi, FR2048 Strasbourg, France.
Université de Strasbourg, CNRS, IPHC UMR7178, Strasbourg, France.
Exp Gerontol. 2022 Jul;164:111811. doi: 10.1016/j.exger.2022.111811. Epub 2022 Apr 25.
In humans, hyperglycemia is associated with protein glycation, which may contribute to aging. Strikingly, birds usually outlive mammals of the same body mass, while exhibiting high plasma glucose levels. However, how birds succeed in escaping pro-aging effects of glycation remains unknown. Using a specific mass spectrometry-based approach in captive zebra finches of known age, we recorded high glycaemia values but no glycated hemoglobin form was found. Still, we showed that zebra finch hemoglobin can be glycated in vitro, albeit only to a limited extent compared to its human homologue. This may be due to peculiar structural features, as supported by the unusual presence of three different tetramer populations with balanced proportions and a still bound cofactor that could be inositol pentaphosphate. High levels of the glycated forms of zebra finch plasma serotransferrin, carbonic anhydrase 2, and albumin were measured. Glucose, age or body mass correlations with either plasma glycated proteins or hemoglobin isoforms suggest that those variables may be future molecular tools of choice to monitor glycation and its link with individual fitness. Our molecular advance may help determine how evolution succeeded in associating flying ability, high blood glucose and long lifespan in birds.
在人类中，高血糖与蛋白质糖化有关，这可能导致衰老。引人注目的是，鸟类通常比同一体重的哺乳动物寿命更长，同时表现出高血糖水平。然而，鸟类如何成功逃脱糖化的衰老效应仍然未知。在已知年龄的圈养斑马雀中使用特定的基于质谱的方法，我们记录了高血糖值，但没有发现糖化血红蛋白形式。尽管如此，我们表明斑马雀血红蛋白可以在体外糖化，尽管与人类同源物相比，糖化程度有限。这可能是由于特殊的结构特征，这得到了三种不同四聚体群体的不同寻常存在的支持，这些群体的比例平衡，并且仍然结合有肌醇五磷酸作为辅因子。测量了斑马雀血浆转铁蛋白、碳酸酐酶 2 和白蛋白的糖化形式的高水平。葡萄糖、年龄或体重与血浆糖化蛋白或血红蛋白同工型的相关性表明，这些变量可能是未来监测糖化及其与个体适应性联系的首选分子工具。我们的分子进展可能有助于确定进化是如何成功地将飞行能力、高血糖和长寿命联系起来的。
