Harrison Paul, Price Joshua, Didembourg Marie, Johnson Alan, Baldwin Samuel, Veronneau Marcel, Baertlein Daniel, Shi Xiaoyong, Machin Samuel
Institute of Inflammation and Ageing, College of Medical and Dental Sciences, University of Birmingham Medical School, Birmingham, UK.
Biotechne, Minneapolis, MN, USA.
Platelets. 2022 Nov 17;33(8):1159-1167. doi: 10.1080/09537104.2022.2060956. Epub 2022 Apr 26.
We have evaluated a commercial-fixed porcine platelet preparation (with and without added fixed human red blood cells (RBC)) for the potential standardization of mean platelet volume (MPV) measurements. The standards (Biotechne) were distributed internationally to 19 laboratories including all major hematology instrument manufacturers and academic/pathology laboratories. Overall, the standards demonstrated excellent stability up to 1 month within both MPV values and platelet counts when stored at 4°C. The presence of RBC significantly increased the platelet count and MPV values compared to platelets alone. However, as expected, there were differences in MPV values between different instruments and manufacturers. MPV values were also significantly higher in the whole blood standard compared to the platelet standard in the majority of instruments except with some instruments, where MPV values were significantly higher in the platelet only preparation. To further investigate this phenomenon, two different Platelet MPV preparations (with low and high MPV) in combination with 3 different RBC MCV preparations (with low, normal or high MCVs) were tested to try and further elucidate how RBC populations may impact upon platelet analysis (count, MPV, and PDW) using a single impedance analyzer. Both MPV and MCV values showed good stability over the course of the study for up to 50 days. As expected, the RBC preparation with the lowest MCV had the greatest impact on the MPV. However, this was not observed with an increase in MCV of the RBC or by a larger MPV of the platelet population. To further understand how different gating strategies may also influence results, we investigated the effect of either fixed or floating gate strategies upon MPV raw data from patient samples in a single impedance analyzer. Overall, it was clear that floating and fixed gate strategies also significantly impact upon MPV values. In conclusion, we have demonstrated the potential of an MPV standard with good stability characteristics for calibrating and comparing full blood counters that use different analysis principles, gating and MPV calculations. This may facilitate future instrument calibration and harmonization of results between different technologies.
我们评估了一种商业化固定猪血小板制剂（添加和不添加固定人红细胞（RBC））用于平均血小板体积（MPV）测量潜在标准化的情况。这些标准品（Biotechne）在国际上分发给了19个实验室，包括所有主要血液学仪器制造商以及学术/病理学实验室。总体而言，当储存在4°C时，这些标准品在MPV值和血小板计数方面均表现出长达1个月的出色稳定性。与单独的血小板相比，RBC的存在显著增加了血小板计数和MPV值。然而，正如预期的那样，不同仪器和制造商之间的MPV值存在差异。除了一些仪器外，大多数仪器中全血标准品的MPV值也显著高于血小板标准品，而在仅含血小板的制剂中，某些仪器的MPV值显著更高。为了进一步研究这一现象，我们测试了两种不同的血小板MPV制剂（低MPV和高MPV）与3种不同的RBC平均红细胞体积（MCV）制剂（低、正常或高MCV）的组合情况，以尝试进一步阐明RBC群体如何使用单一阻抗分析仪影响血小板分析（计数、MPV和血小板分布宽度（PDW））。在长达50天的研究过程中，MPV和MCV值均显示出良好的稳定性。正如预期的那样，MCV最低的RBC制剂对MPV的影响最大。然而，随着RBC的MCV增加或血小板群体的MPV增大，并未观察到这种情况。为了进一步了解不同的门控策略如何影响结果，我们在单一阻抗分析仪中研究了固定或浮动门控策略对患者样本MPV原始数据的影响。总体而言，很明显浮动和固定门控策略也会对MPV值产生显著影响。总之，我们已经证明了具有良好稳定性特征的MPV标准品在校准和比较使用不同分析原理、门控和MPV计算的全血细胞计数器方面的潜力。这可能有助于未来的仪器校准以及不同技术之间结果的统一。
