Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, China.
J Biophotonics. 2022 Aug;15(8):e202200061. doi: 10.1002/jbio.202200061. Epub 2022 May 3.
The use of skin flaps to fill large defects is a key surgical technique in reconstructive surgery, effective real-time in vivo imaging for flap design and use is urgent. Currently, fluorescent imaging in the second NIR window (NIR-II; 1000-1700 nm) is characterized by non-radiation, less expensive and higher resolution in comparisons with the first NIR window (NIR-I; 700-900 nm) and other traditional imaging modalities. In this article, we identified the location and numbers of perforators and choke zone via NIR-II imaging. Then, eight abdominal perforator flaps were established and the perfusion zones were evaluatedat special time points. Finally, after eight pedicled flaps establishment, NIR-II imaging was used to guide the optimal timing for division of flap pedicle. The results showed that NIR-II fluorescence imaging with indocyanine green (ICG) can reliably visualize vascular supply, which makes it to be an accurate and in vivo imaging approach to flap clinical design and use.
皮瓣用于填充大面积缺损是重建外科的关键技术，因此对于皮瓣设计和使用的有效实时体内成像技术需求迫切。目前，与第一近红外窗口（NIR-I；700-900nm）和其他传统成像方式相比，第二近红外窗口（NIR-II；1000-1700nm）的荧光成像具有非辐射、成本更低、分辨率更高的特点。在本文中，我们通过近红外二区（NIR-II）成像来确定穿支血管的位置和数量以及梗塞区。然后，建立了 8 个腹部穿支皮瓣，并在特定时间点评估灌注区。最后，在建立 8 个带蒂皮瓣后，使用近红外二区（NIR-II）成像来指导皮瓣蒂部的最佳分离时机。结果表明，吲哚菁绿（ICG）的近红外二区（NIR-II）荧光成像可以可靠地显示血管供应，这使其成为一种用于皮瓣临床设计和使用的准确、实时的体内成像方法。
