Gao Shanshan, Yang Gege, Zhang Xiaohui, Lu Ying, Chen Ying, Wu Xiangwei, Song Chunxia
Department of Applied Chemistry, School of Science, Anhui Agricultural University, Hefei 230036, China.
College of Resources and Environment, Key Laboratory of Agri-food Safety of Anhui Province, Anhui Agricultural University, Hefei 230036, China.
ACS Omega. 2022 Apr 8;7(15):12747-12752. doi: 10.1021/acsomega.1c07295. eCollection 2022 Apr 19.
The extensive use of organophosphorus pesticides in agriculture poses a high risk to human health and has boosted the demands for developing sensitive monitoring methods. Herein, we developed a facile and sensitive method for isocarbophos detection based on the remarkable fluorescence enhancement of pyrene during host-guest interaction of β-cyclodextrin polymer (β-CDP) and pyrene. The 3'-pyrene-labeled isocarbophos aptamer could be cleaved by exonuclease I to obtain free pyrene that was tagged on mononucleotides, which could enter the hydrophobic cavity of β-CDP, resulting in a prominent fluorescence enhancement. While the target isocarbophos was added, aptamer could undergo a conformational change into a hairpin complex, which prevented the cleavage and host-guest interaction because of the steric hindrance, leading to a weak fluorescence. The isocarbophos has been sensitively and selectively analyzed by detecting the system fluorescence intensity with a detection limit as low as 1.2 μg/L. In addition, we have verified the ability of our proposed method in real sample detection from fruit extract.
农业中有机磷农药的广泛使用对人类健康构成了高风险，并推动了对开发灵敏监测方法的需求。在此，我们基于β-环糊精聚合物（β-CDP）与芘的主客体相互作用过程中芘显著的荧光增强，开发了一种简便灵敏的水胺硫磷检测方法。3'-芘标记的水胺硫磷适配体可被核酸外切酶I切割，以获得标记在单核苷酸上的游离芘，其可进入β-CDP的疏水腔，导致显著的荧光增强。当加入目标水胺硫磷时，适配体可发生构象变化形成发夹复合物，由于空间位阻，这会阻止切割和主客体相互作用，导致荧光减弱。通过检测体系荧光强度，已对水胺硫磷进行了灵敏且选择性的分析，检测限低至1.2 μg/L。此外，我们已经验证了所提出方法在水果提取物实际样品检测中的能力。
