Faculty of Science, Hokkaido University, Sapporo, Japan.
Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo, Japan.
Methods Mol Biol. 2022;2430:193-203. doi: 10.1007/978-1-0716-1983-4_12.
In vitro gliding assay of the filamentous protein microtubule (MT) on a kinesin motor protein coated surface has appeared as a classic platform for studying active matters. At high densities, the gliding MTs spontaneously align and self-organize into fascinating large-scale patterns. Application of mechanical stimuli e.g., stretching stimuli to the MTs gliding on a kinesin-coated surface can modulate their self-organization and patterns according to the boundary conditions. Depending on the mode of stretching, MT at high densities change their moving direction and exhibit various kinds of patterns such as stream, zigzag and vortex pattern. In this chapter, we discuss detail procedures on how to apply mechanical stimuli to the moving MTs on a kinesin coated substrate.
在体外丝状蛋白微管（MT）在驱动蛋白马达蛋白涂覆表面上的滑行实验已成为研究活性物质的经典平台。在高密度下，滑行的 MT 会自发地对齐并自我组织成迷人的大规模图案。施加机械刺激，例如对在驱动蛋白涂覆表面上滑行的 MT 施加拉伸刺激，可以根据边界条件来调节它们的自组织和图案。根据拉伸的方式，高密度的 MT 会改变它们的运动方向，并表现出各种图案，如流型、锯齿型和涡旋型。在本章中，我们详细讨论了如何向驱动蛋白涂覆基底上运动的 MT 施加机械刺激的方法。
