用于微管蛋白-配体复合物高分辨率结构分析的结晶系统。

Crystallization Systems for the High-Resolution Structural Analysis of Tubulin-Ligand Complexes.

机构信息

Laboratory of Biomolecular Research, Division of Biology and Chemistry, Paul Scherrer Institut, Villigen PSI, Switzerland.

University of Basel, Biozentrum, Basel, Switzerland.

出版信息

Methods Mol Biol. 2022;2430:349-374. doi: 10.1007/978-1-0716-1983-4_23.

DOI:10.1007/978-1-0716-1983-4_23

PMID:35476344

Abstract

Since the first moderate resolution, structural description of Taxol bound to tubulin by electron crystallography in 1998, several tubulin crystal systems have been developed and optimized for the high-resolution analysis of tubulin-ligand complexes by X-ray crystallography. Here we describe three tubulin crystal systems that have allowed investigating the molecular mechanisms of action of a large number of diverse anti-tubulin agents.

摘要

自 1998 年首次通过电子晶体学对紫杉醇与微管蛋白结合进行中等分辨率的结构描述以来，已经开发并优化了几种微管蛋白晶体系统，以通过 X 射线晶体学对微管蛋白-配体复合物进行高分辨率分析。在这里，我们描述了三种微管蛋白晶体系统，这些系统允许研究大量不同的抗微管蛋白药物的作用机制。