Magny Romain, Regazzetti Anne, Kessal Karima, Christin Orane, Baudouin Christophe, Roulland Emmanuel, Brignole-Baudouin Françoise, Laprévote Olivier, Auzeil Nicolas
Sorbonne Université UM80, INSERM UMR 968, CNRS UMR 7210, Institut de la Vision, IHU ForeSight, 75012, Paris, France; Université Paris Cité, CNRS, CiTCoM, F-75006, Paris, France.
Université Paris Cité, CNRS, CiTCoM, F-75006, Paris, France.
Biochimie. 2022 Dec;203:3-10. doi: 10.1016/j.biochi.2022.04.008. Epub 2022 Apr 25.
Three new very long chain polyunsaturated fatty acids (VLC PUFA) belonging to the omega-3 family have been identified in meibum samples collected by Schirmer strips. These VLC PUFA, namely FA (32:3), FA (34:3) and FA (36:3), were detected in O-acyl-ω-hydroxy fatty acids using a molecular network approach, and as free fatty acids. Identification was supported by retention time prediction model, exact mass determination and isotopic patterns. Double bond location was determined using cross metathesis reaction associated to tandem mass spectrometry. In meibum, synthesis of these VLC PUFA is likely to be mediated by elongation of very long chain fatty acids 4 enzyme. The biological role of these newly VLC PUFA and their occurrence in other tissues and biological fluids remains to be elucidated.
