LncRNA TSPEAR-AS1 predicts poor prognosis in patients with hepatitis B virus-associated hepatocellular carcinoma and promotes metastasis via miR-1915-5p.

BACKGROUND

Hepatitis B virus-related hepatocellular carcinoma (HBV-HCC) will contribute to more than half of the liver deaths worldwide. This study aimed to investigate the prognostic value of TSPEAR-AS1 in HBV-HCC and its role in the HBV-HCC progression.

METHODS

HBV-HCC tissue and adjacent non-cancerous tissues (ANT) were detected to figure out the expression level of TSPEAR-AS1 using real-time quantitative PCR. The relationship between TSPEAR-AS1 expression and each important clinical characteristic was evaluated. And the prognostic significance of TSPEAR-AS1 was assessed by Kaplan-Meier curve and Cox regression analysis. CCK-8 and Transwell assays were performed to observe the effects of TSPEAR-AS1 on HBV-HCC cell proliferation, migration, and invasion.

RESULTS

The TSPEAR-AS1 expression was downregulated in HBV-HCC tissues, as well as in HBV-HCC cell lines. The downregulation of TSPEAR-AS1 showed a significant association with TNM stage, clinical stage, and vascular invasion and predicted poor prognosis of HBV-HCC patients. Overexpression of TSPEAR-AS1 inhibited HBV-HCC cell ability of proliferation, migration, and invasiveness. TSPEAR-AS1 may bind to miR-1915-5p in HCC.

CONCLUSION

TSPEAR-AS1 expression was downregulated in HBV-HCC and may serve as a potential prognostic factor. TSPEAR-AS1 might exert a suppressor role in HBV-HCC through inhibiting tumor cell proliferation, migration, and invasion.