Joshi Dharaben J, Malek Naved I, Muthukumaran Rajendra Bose, Kailasa Suresh Kumar
Department of Chemistry, 123518Sardar Vallabhbhai National Institute of Technology, Surat, India.
Department of Chemistry, 29670Mizoram University, Aizawl, India.
Appl Spectrosc. 2022 Oct;76(10):1234-1245. doi: 10.1177/00037028221100544. Epub 2022 Jun 20.
In this work, a microwave assisted method was developed for synthesis of red fluorescent copper nanoclusters (NCs) using trypsin as a template (trypsin-Cu). The as-synthesized trypsin-Cu NCs are stable and water soluble, exhibiting fluorescence emission at 657 nm when excited at 490 nm. The as-prepared red-emitting trypsin-Cu NCs were characterized by using several analytical techniques such as ultraviolet-visible (UV-Vis) and fluorescence, fluorescence lifetime, Fourier transform infrared, and X-ray photoelectron spectroscopic techniques. Red-emitting trypsin-Cu NCs acted as a nanosensor for sensing both Pb and Hg ions through fluorescence quenching. Using this approach, good linearities are observed in the range of 0.1-25 and of 0.001-1 μM with the lower limit of detection of 14.63 and 56.81 nM for Pb and Hg ions, respectively. Trypsin-Cu NCs-based fluorescence assay was successfully applied to detect both Hg and Pb ions in water and tobacco samples.
在本研究中，开发了一种微波辅助方法，以胰蛋白酶为模板合成红色荧光铜纳米簇（NCs）（胰蛋白酶-Cu）。合成的胰蛋白酶-Cu NCs稳定且水溶性良好，在490nm激发时，在657nm处呈现荧光发射。通过紫外可见（UV-Vis）光谱、荧光光谱、荧光寿命、傅里叶变换红外光谱和X射线光电子能谱等多种分析技术对制备的发红光的胰蛋白酶-Cu NCs进行了表征。发红光的胰蛋白酶-Cu NCs通过荧光猝灭作为检测Pb和Hg离子的纳米传感器。使用这种方法，在0.1-25和0.001-1μM范围内观察到良好的线性关系，Pb和Hg离子的检测下限分别为14.63和56.81 nM。基于胰蛋白酶-Cu NCs的荧光分析法成功应用于检测水和烟草样品中的Hg和Pb离子。
