Manyu Li, Xiuhua Zhao, Guojun Liu, Guixue Zhang
College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
Institute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin, China.
Front Vet Sci. 2022 Apr 11;9:874679. doi: 10.3389/fvets.2022.874679. eCollection 2022.
Goose is a seasonal breeding animal. Its reproduction is regulated by hypothalamus-pituitary-gonad axis and also affected by environmental factors such as light and location. Zi-goose is characterized with long egg-laying period and high egg-laying potential and belongs to the long-day type of seasonal breeding. In this study, the regulation mechanism of different lighting on reproductive performance of Zi-goose by using metabonomics analysis technology. In addition, 1,481 differential metabolites were screened out totally. 583 differential metabolites were identification in hypothalamus of Zi-goose. 196 differential metabolites were identification in pituitary of Zi-goose. 692 differential metabolites were identification in ovary of Zi-goose. Under red light condition for 12 h, expression of 433 differential metabolites were down-regulated and expression of 150 differential metabolites were up regulated in hypothalamus of Zi-goose, expression of 125 differential metabolites were down-regulated and expression of 71 differential metabolites were up-regulated in pituitary of Zi-goose, expression of 355 differential metabolites were down-regulated and expression of 337 differential metabolites were up-regulated in ovary of Zi-goose. 33 differential metabolites were closely associated with 1,264 transcripts and 400 homologous genes of related enzymes in hypothalamus of Zi-goose. 15 differential metabolites were closely associated with 163 transcripts and 47 homologous genes of related enzymes in pituitary of Zi-goose. 55 differential metabolites were closely associated with 1,255 transcripts and 360 homologous genes of related enzymes in ovary of Zi-goose. It was confirmed that four metabolic pathways were closely related to light regulation of reproductive performance of Zi-goose, namely GnRH signaling pathway, prolactin signaling pathway, thyroid hormone synthesis and ovarian steroidogenesis. Typical differential metabolites of arachidonic acid, glucose-6-phosphate, progesterone, glutathione, oxidized glutathione, testosterone, deoxyepiandrosterone and their related protein genes would play an important role in light regulation of reproductive performance of Zi-goose.
鹅是季节性繁殖动物。其繁殖受下丘脑 - 垂体 - 性腺轴调控，同时也受光照和地理位置等环境因素影响。籽鹅产蛋期长、产蛋潜力高，属于季节性繁殖的长日照类型。本研究采用代谢组学分析技术探讨不同光照对籽鹅繁殖性能的调控机制。此外，共筛选出1481种差异代谢物。在籽鹅下丘脑鉴定出583种差异代谢物，在垂体鉴定出196种差异代谢物，在卵巢鉴定出692种差异代谢物。在12小时红光条件下，籽鹅下丘脑中有433种差异代谢物表达下调，150种差异代谢物表达上调；垂体中有125种差异代谢物表达下调，71种差异代谢物表达上调；卵巢中有355种差异代谢物表达下调，337种差异代谢物表达上调。33种差异代谢物与籽鹅下丘脑1264个转录本及相关酶的400个同源基因密切相关。15种差异代谢物与籽鹅垂体163个转录本及相关酶的47个同源基因密切相关。55种差异代谢物与籽鹅卵巢1255个转录本及相关酶的360个同源基因密切相关。证实了四条代谢途径与籽鹅繁殖性能的光照调控密切相关，即促性腺激素释放激素信号通路、催乳素信号通路、甲状腺激素合成和卵巢类固醇生成。花生四烯酸、6 - 磷酸葡萄糖、孕酮、谷胱甘肽、氧化型谷胱甘肽、睾酮、脱氢表雄酮等典型差异代谢物及其相关蛋白基因在籽鹅繁殖性能的光照调控中发挥重要作用。
