ImmunoEngineering Lab, School of Cancer and Pharmaceutical Sciences, King's College London, Guy's Cancer Centre, Great Maze Pond, London SE1 9RT, UK.
Department Infectious Diseases, School of Immunology and Microbial Sciences, Guy's Campus, King's College London, London SE1 9RT, UK.
STAR Protoc. 2022 Mar 22;3(2):101300. doi: 10.1016/j.xpro.2022.101300. eCollection 2022 Jun 17.
The gold standard protocol for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection detection remains reverse transcription quantitative polymerase chain reaction (qRT-PCR), which detects viral RNA more sensitively than any other approach. Here, we present Homebrew, a low-cost protocol to extract RNA using widely available reagents. Homebrew is as sensitive as commercially available RNA extraction kits. Homebrew allows for sample pooling and can be adapted for automation in high-throughput settings. For complete details on the use and execution of this protocol, please refer to Page et al. (2022).
用于严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）感染检测的金标准方案仍然是逆转录定量聚合酶链反应（qRT-PCR），该方法比任何其他方法都更灵敏地检测病毒 RNA。在这里，我们提出了 Homebrew，这是一种使用广泛可用的试剂提取 RNA 的低成本方案。Homebrew 与市售 RNA 提取试剂盒一样灵敏。Homebrew 允许样本混合，并可适应高通量环境中的自动化。有关此方案的使用和执行的完整详细信息，请参阅 Page 等人（2022 年）。
