Moore F M, White S D, Carpenter J L, Torchon E
Vet Immunol Immunopathol. 1987 Jan;14(1):1-9. doi: 10.1016/0165-2427(87)90070-5.
Formalin-fixed, paraffin embedded skin biopsy specimens from 44 dogs with various dermatopathies were examined for the presence of immunoglobulins (IgG, IgM, IgA) and complement (C3) by the peroxidase antiperoxidase method (PAP). Final diagnoses of the skin diseases were determined by clinical findings, fungal and bacterial cultures, skin scrapings, serum endocrinologic tests, and histologic features of cutaneous biopsies. The dermatopathies included examples of pyoderma (folliculitis/furunculosis), demodecosis, sarcoptic mange, dermatophytosis, endocrine dermatopathy, and autoimmune skin disease (AISD). In the latter category, 7 cases of pemphigus foliaceus, 1 pemphigus vulgaris, 4 discoid lupus erythematosus (DLE) and 4 examples of indeterminate AISD were examined. Immunoglobulins, C3, or both, were localized in tissue sections from AISD (15/16), pyoderma (7/11), demodecosis (4/8) sarcoptic mange (3/3), and dermatomycosis (2/3). Endocrine dermatopathy biopsies were consistently negative for Ig and C3 (0/3). The pattern of localization was most often intercellular (31/44 positive biopsies) with basement membrane immunoreactivity in 4 cases of DLE, and 1 case of indeterminate AISD. There was no consistent correlation between histologic features of hematoxylin and eosin-stained biopsies and the presence of Ig and C3. Clinical outcome was similar in both Ig and C3 positive and negative cases of non-AISD dermatitis. The high percentage (95%) of positive results in AISD biopsies indicates the usefulness and sensitivity of the PAP method for the localization of Ig and C3. Because of the high percentage of Ig localization in pyoderma (73%), biopsy specimens with extensive inflammatory skin disease are not suitable for diagnosis of AISD. The PAP method is useful in the diagnosis of AISD in biopsy specimens with minimal inflammation. Caution is warranted in the interpretation of immunoreactivity independent of clinical and histologic information.
采用过氧化物酶抗过氧化物酶法（PAP），对44只患有各种皮肤病的犬的福尔马林固定、石蜡包埋皮肤活检标本进行免疫球蛋白（IgG、IgM、IgA）和补体（C3）检测。皮肤病的最终诊断依据临床表现、真菌和细菌培养、皮肤刮片、血清内分泌检测以及皮肤活检的组织学特征来确定。皮肤病包括脓疱病（毛囊炎/疖病）、蠕形螨病、疥螨病、皮肤癣菌病、内分泌性皮肤病和自身免疫性皮肤病（AISD）。在后者中，检查了7例落叶型天疱疮、1例寻常型天疱疮、4例盘状红斑狼疮（DLE）和4例不确定的AISD。免疫球蛋白、C3或两者在AISD（15/16）、脓疱病（7/11）、蠕形螨病（4/8）、疥螨病（3/3）和皮肤癣菌病（2/3）的组织切片中定位。内分泌性皮肤病活检标本的Ig和C3始终为阴性（0/3）。定位模式最常见的是细胞间（31/44例阳性活检），4例DLE和1例不确定的AISD病例出现基底膜免疫反应性。苏木精和伊红染色活检的组织学特征与Ig和C3的存在之间没有一致的相关性。非AISD性皮炎的Ig和C3阳性和阴性病例的临床结果相似。AISD活检中高比例（95%）的阳性结果表明PAP法对Ig和C3定位的有用性和敏感性。由于脓疱病中Ig定位的高比例（73%），患有广泛炎症性皮肤病的活检标本不适合诊断AISD。PAP法有助于诊断炎症轻微的活检标本中的AISD。在独立于临床和组织学信息解释免疫反应性时需谨慎。
Zotero 插件